Relationship between Microbial Translocation and Endothelial Function in HIV Infected Patients

Emily Blodget, Changyu Shen, Grace Aldrovandi, Adrienne Rollie, Samir Gupta, James H. Stein, Michael P. Dubé

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Background: Circulating levels of microbial products are increased in HIV infection, and provoke endothelial dysfunction in other disease settings. Methodology/Principal Findings: We examined data from a cross-sectional single site study at Indiana University (Indiana, N = 85) and a 24- week multicenter prospective study of antiretroviral therapy (ART) initiation (ACTG 5152s, N = 75). Brachial artery flow-mediated dilation (FMD) was measured by ultrasound. Plasma lipopolysaccharide (LPS) and soluble CD14 (sCD14) levels were measured from stored specimens and correlated with FMD values using Pearson correlations. The Indiana subjects were 63% male with a mean age of 39 years and a median CD4 count of 406 cells/mm3 (388 not on ART, 464 on ART). The 5152s subjects were 92% were male with a mean age of 35 years and a median CD4 count of 251 cells/mm3 at entry which increased to 396 cells/mm3 on ART. When analyzing the two cohorts individually or in combination neither sCD14 nor LPS correlated significantly with FMD. In a pre-specified subgroup analysis of the Indiana subjects receiving ART (N = 46, mean ART duration 40 months) LPS was inversely correlated with FMD (r = -0.33, p = 0.02), but not sCD14 (r = -0.01, p = 0.9). Multivariate analysis confirmed LPS as an independent predictor of FMD in this subgroup (p = 0.02). Conclusions/Significance: In HIV- infected individuals on prolonged ART, higher LPS levels are associated with worse endothelial function but not in untreated subjects or at 24 weeks after ART initiation. Persistent microbial translocation may contribute to arterial dysfunction and the increased cardiovascular disease risk observed in individuals on long-term ART.

Original languageEnglish
Article numbere42624
JournalPLoS One
Volume7
Issue number8
DOIs
StatePublished - Aug 30 2012

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Lipopolysaccharides
HIV
therapeutics
lipopolysaccharides
Dilatation
Therapeutics
CD4 Lymphocyte Count
Ultrasonics
Plasmas
Brachial Artery
HIV infections
cells
prospective studies
arteries
cardiovascular diseases
multivariate analysis
Multicenter Studies
HIV Infections
Cardiovascular Diseases
Multivariate Analysis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Blodget, E., Shen, C., Aldrovandi, G., Rollie, A., Gupta, S., Stein, J. H., & Dubé, M. P. (2012). Relationship between Microbial Translocation and Endothelial Function in HIV Infected Patients. PLoS One, 7(8), [e42624]. https://doi.org/10.1371/journal.pone.0042624

Relationship between Microbial Translocation and Endothelial Function in HIV Infected Patients. / Blodget, Emily; Shen, Changyu; Aldrovandi, Grace; Rollie, Adrienne; Gupta, Samir; Stein, James H.; Dubé, Michael P.

In: PLoS One, Vol. 7, No. 8, e42624, 30.08.2012.

Research output: Contribution to journalArticle

Blodget, E, Shen, C, Aldrovandi, G, Rollie, A, Gupta, S, Stein, JH & Dubé, MP 2012, 'Relationship between Microbial Translocation and Endothelial Function in HIV Infected Patients', PLoS One, vol. 7, no. 8, e42624. https://doi.org/10.1371/journal.pone.0042624
Blodget, Emily ; Shen, Changyu ; Aldrovandi, Grace ; Rollie, Adrienne ; Gupta, Samir ; Stein, James H. ; Dubé, Michael P. / Relationship between Microbial Translocation and Endothelial Function in HIV Infected Patients. In: PLoS One. 2012 ; Vol. 7, No. 8.
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