Relationship between topoisomerase 2A RNA expression and recurrence after adjuvant chemotherapy for breast cancer

Joseph A. Sparano, Lori J. Goldstein, Barrett H. Childs, Steven Shak, Diana Brassard, Sunil Badve, Frederick L. Baehner, Roberto Bugarini, Steve Rowley, Edith Perez, Lawrence N. Shulman, Silvana Martino, Nancy E. Davidson, George W. Sledge, Robert Gray

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Purpose: To perform an exploratory analysis of the relationship between gene expression and recurrence in operable hormone receptor (HR)-positive, human epidermal growth factor receptor 2 (HER2)-normal breast cancer patients treated with adjuvant doxorubicin-containing chemotherapy. Experimental Design: RNA was extracted from archived tumor samples derived from 378 patients with stage I to III HR-positive, HER2-normal breast cancer and analyzed by reverse transcription-PCR for a panel of 374 genes, including the 21-gene recurrence score (RS). Patients were randomized to receive adjuvant doxorubicin plus cyclophosphamide or docetaxel in trial E2197, with no difference in recurrence seen in the treatment arms. All available recurrent cases were selected plus a nonrecurrent cohort. Cox proportional hazard models were used to identify relationships between gene expression and recurrence. Results: TOP2A expression exhibited the strongest association with increased recurrence risk (P = 0.01), and was significantly associated with recurrence (P = 0.008) in a multivariate analysis adjusted for clinicopathologic features. Elevated TOP2A expression above the median was associated with a 2.6-fold increase (95% confidence interval, 1.3-5.2; P = 0.008) in risk of recurrence if the RS was

Original languageEnglish (US)
Pages (from-to)7693-7700
Number of pages8
JournalClinical Cancer Research
Volume15
Issue number24
DOIs
StatePublished - Dec 15 2009
Externally publishedYes

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Adjuvant Chemotherapy
RNA
Breast Neoplasms
Recurrence
docetaxel
Doxorubicin
Hormones
Gene Expression
Proportional Hazards Models
Cyclophosphamide
Genes
Reverse Transcription
Research Design
Multivariate Analysis
Confidence Intervals
Drug Therapy
Polymerase Chain Reaction
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Relationship between topoisomerase 2A RNA expression and recurrence after adjuvant chemotherapy for breast cancer. / Sparano, Joseph A.; Goldstein, Lori J.; Childs, Barrett H.; Shak, Steven; Brassard, Diana; Badve, Sunil; Baehner, Frederick L.; Bugarini, Roberto; Rowley, Steve; Perez, Edith; Shulman, Lawrence N.; Martino, Silvana; Davidson, Nancy E.; Sledge, George W.; Gray, Robert.

In: Clinical Cancer Research, Vol. 15, No. 24, 15.12.2009, p. 7693-7700.

Research output: Contribution to journalArticle

Sparano, JA, Goldstein, LJ, Childs, BH, Shak, S, Brassard, D, Badve, S, Baehner, FL, Bugarini, R, Rowley, S, Perez, E, Shulman, LN, Martino, S, Davidson, NE, Sledge, GW & Gray, R 2009, 'Relationship between topoisomerase 2A RNA expression and recurrence after adjuvant chemotherapy for breast cancer', Clinical Cancer Research, vol. 15, no. 24, pp. 7693-7700. https://doi.org/10.1158/1078-0432.CCR-09-1450
Sparano, Joseph A. ; Goldstein, Lori J. ; Childs, Barrett H. ; Shak, Steven ; Brassard, Diana ; Badve, Sunil ; Baehner, Frederick L. ; Bugarini, Roberto ; Rowley, Steve ; Perez, Edith ; Shulman, Lawrence N. ; Martino, Silvana ; Davidson, Nancy E. ; Sledge, George W. ; Gray, Robert. / Relationship between topoisomerase 2A RNA expression and recurrence after adjuvant chemotherapy for breast cancer. In: Clinical Cancer Research. 2009 ; Vol. 15, No. 24. pp. 7693-7700.
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