Relationship of colony stimulating activity to apparent kill of human colony forming cells by irradiation and hydroxyurea

H. E. Broxmeyer, P. R. Galbraith, F. L. Baker

Research output: Contribution to journalArticle

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Abstract

Suspensions of human bone marrow cells were subjected to 137Cs irradiation in vitro and then cultured in semisolid agar medium. Cultures of irradiated cells were stimulated with colony stimulating activity (CSA) of different potencies, and it was found that the amount of stimulation applied to cultures influenced the apparent kill of colony forming cells (CFC). It was also found that the effects of irradiation on colony formation were not confined to CFC kill since medium conditioned by cells during irradiation exhibited stimulatory and inhibitory properties after treatment by 600 and 1000 rads, respectively. Studies in which irradiated cells were pretreated with hydroxyurea indicated that CFC in the DNA synthetic phase of the cell cycle were particularly sensitive to low doses of irradiation. The proliferative capacity of CFC surviving 1000 rads was undiminished as judged by their ability to form large colonies. Estimates of CFC kill by hydroxyurea were also affected by the level of CSA.

Original languageEnglish (US)
Pages (from-to)403-411
Number of pages9
JournalBlood
Volume47
Issue number3
StatePublished - Dec 1 1976

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Hydroxyurea
Cells
Irradiation
Conditioned Culture Medium
Cell culture
Dosimetry
Agar
Suspensions
Bone
Artificial Cells
DNA
Bone Marrow Cells
Cell Cycle
Cell Culture Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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Relationship of colony stimulating activity to apparent kill of human colony forming cells by irradiation and hydroxyurea. / Broxmeyer, H. E.; Galbraith, P. R.; Baker, F. L.

In: Blood, Vol. 47, No. 3, 01.12.1976, p. 403-411.

Research output: Contribution to journalArticle

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