Relaxation of arterial smooth muscle by calcium sparks

M. T. Nelson, H. Cheng, M. Rubart, L. F. Santana, A. D. Bonev, H. J. Knot, W. J. Lederer

1094 Scopus citations

Abstract

Local increases in intracellular calcium ion concentration ([Ca2+](i)) resulting from activation of the ryanodine-sensitive calcium-release channel in the sarcoplasmic reticulum (SR) of smooth muscle cause arterial dilation. Ryanodine-sensitive, spontaneous local increases in [Ca2+](i) (Ca2+ sparks) from the SR were observed just under the surface membrane of single smooth muscle cells from myogenic cerebral arteries. Ryanodine and thapsigargin inhibited Ca2+ sparks and Ca2+-dependent potassium (K(Ca)) currents, suggesting that Ca2+ sparks activate K(Ca) channels. Furthermore, K(Ca) channels activated by Ca2+ sparks appeared to hyperpolarize and dilate pressurized myogenic arteries because ryanodine and thapsigargin depolarized and constricted these arteries to an extent similar to that produced by blockers of K(Ca) channels. Ca2+ sparks indirectly cause vasodilation through activation of K(Ca) channels, but have little direct effect on spatially averaged [Ca2+](i), which regulates contraction.

Original languageEnglish (US)
Pages (from-to)633-637
Number of pages5
JournalScience
Volume270
Issue number5236
DOIs
StatePublished - Jan 1 1995

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Nelson, M. T., Cheng, H., Rubart, M., Santana, L. F., Bonev, A. D., Knot, H. J., & Lederer, W. J. (1995). Relaxation of arterial smooth muscle by calcium sparks. Science, 270(5236), 633-637. https://doi.org/10.1126/science.270.5236.633