Release of CuPTSM from human serum albumin after addition of fatty acids

Hua Yuan, William E. Antholine, Witold K. Subczynski, Mark Green

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Copper-62 labeled pyruvaldehyde bis (N4-methyl-thiosemicarbazonato) copper(II), CuPTSM, has been used to probe tissue perfusion by means of positron emission tomograpby. Despite promising results from animals, problems have been encountered in the use of 62CuPTSM to quantitate regional myocardial blood flow in humans. Ultrafiltration and plasma/erythrocyte partitioning studies with radiotracer have previously shown that CuPTSM is bound much more strongly by human serum albumin (HSA) than by dog serum albumin (DSA), limiting its ability to freely diffuse from blood into tissue. In this study, it is confirmed by electron spin resonance (ESR) that CuPTSM strongly binds to HSA with an apparent g(is parallel with) value of 2.12 and an apparent A(is parallel with) value of 186 G. It is also shown that both spin-labeled stearic acid (5-SASL) and nonspin-labeled stearate inhibit CuPTSM binding to HSA. CuPTSM is completely released from HSA when the ratio of 5-SASL to HSA is 5:1. When pure sodium stearate is used, the binding of CuPTSM significantly decreased, about 73% of CuPTSM is released with a ratio of 4:1 stearate to HSA. These results highlight a means of liberating CuPTSM from HSA.

Original languageEnglish (US)
Pages (from-to)251-259
Number of pages9
JournalJournal of Inorganic Biochemistry
Volume61
Issue number4
DOIs
StatePublished - Mar 1996
Externally publishedYes

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Serum Albumin
Fatty Acids
Stearates
Copper
Blood
Tissue
Pyruvaldehyde
Regional Blood Flow
Electron Spin Resonance Spectroscopy
Ultrafiltration
Positrons
Paramagnetic resonance
Animals
Perfusion
Erythrocytes
Dogs
Electrons
Plasmas

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

Cite this

Release of CuPTSM from human serum albumin after addition of fatty acids. / Yuan, Hua; Antholine, William E.; Subczynski, Witold K.; Green, Mark.

In: Journal of Inorganic Biochemistry, Vol. 61, No. 4, 03.1996, p. 251-259.

Research output: Contribution to journalArticle

Yuan, Hua ; Antholine, William E. ; Subczynski, Witold K. ; Green, Mark. / Release of CuPTSM from human serum albumin after addition of fatty acids. In: Journal of Inorganic Biochemistry. 1996 ; Vol. 61, No. 4. pp. 251-259.
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