Removal of sodium channel inactivation in squid giant axons by N-bromoacetamide

G. S. Oxford, C. H. Wu, T. Narahashi

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

The group-specific protein reagents, N-bromoacetamide (NBA) ad N-bromosuccinimide (NBS), modify sodium channel gating when perfused inside squid axons. The normal fast inactivation of sodium channels is irreversibly destroyed by 1 mM NBA or NBS near neutral pH. NBA apparently exhibits an all-manner similar to internal perfusion of Pronase. Despite the complete removal of inactivation by NBA, the voltage-dependent activation of sodium channels remains unaltered as determined by sodium current turn-on kinetics, sodium tail current kinetics, voltage dependence of steady-state activation, and sensitivity of sodium channels to external calcium concentration. NBA and NBS, which can cleave peptide bonds only at tryptophan, tyrosine, or histidine residues and can oxidize sulfur-containing amino acids, were directly compared with regard to effects on sodium inactivation to several other reagent exhibiting overlapping only other compound examined capable of partially mimicking NBA. Our results are consistent with recent models of sodium inactivation and support the involvement of a tyrosine residue in the inactivation gating structure of the sodium channel.

Original languageEnglish (US)
Pages (from-to)227-247
Number of pages21
JournalJournal of General Physiology
Volume71
Issue number3
StatePublished - 1978
Externally publishedYes

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Decapodiformes
Sodium Channels
Axons
Bromosuccinimide
Sodium
Tyrosine
Sulfur Amino Acids
Pronase
Histidine
Tryptophan
N-bromoacetamide
Tail
Perfusion
Calcium
Peptides
Proteins

ASJC Scopus subject areas

  • Physiology

Cite this

Removal of sodium channel inactivation in squid giant axons by N-bromoacetamide. / Oxford, G. S.; Wu, C. H.; Narahashi, T.

In: Journal of General Physiology, Vol. 71, No. 3, 1978, p. 227-247.

Research output: Contribution to journalArticle

Oxford, GS, Wu, CH & Narahashi, T 1978, 'Removal of sodium channel inactivation in squid giant axons by N-bromoacetamide', Journal of General Physiology, vol. 71, no. 3, pp. 227-247.
Oxford, G. S. ; Wu, C. H. ; Narahashi, T. / Removal of sodium channel inactivation in squid giant axons by N-bromoacetamide. In: Journal of General Physiology. 1978 ; Vol. 71, No. 3. pp. 227-247.
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