Ren-1 and Ren-2 loci are expressed in mouse kidney

Loren Field, K. W. Gross

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Inbred strains of mice can be categorized into two groups based on the absence or presence of a duplicated copy of the renin structural gene; one-gene strains carry a single renin gene (Ren-1), whereas two-gene strains carry two renin genes (Ren-1 and Ren-2). To investigate the contribution that each locus makes to the composite levels of renin mRNA observed to accumulate in different tissues of two-gene strains, we have developed two assays capable of distinguishing the highly homologous Ren-1 and Ren-2 transcripts. Both methods take advantage of established base sequence differences between Ren-1 and Ren-2 coding regions by using reverse transcriptase-mediated primer extension of oligonucleotide primer/mRNA hybrids in the presence of appropriate deoxy- and dideoxynucleotide phosphates. Using these techniques we found that Ren-1 and Ren-2 mRNAs accumulate in the kidney of two-gene strains to approximately equal levels. These observations are discussed in light of potential mechanisms regulating the tissue-specific expression of the Ren-1 and Ren-2 loci.

Original languageEnglish (US)
Pages (from-to)6196-6200
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number18
StatePublished - 1985
Externally publishedYes

Fingerprint

Kidney
Renin
Genes
Messenger RNA
Dideoxynucleotides
Inbred Strains Mice
DNA Primers
RNA-Directed DNA Polymerase
Phosphates

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Ren-1 and Ren-2 loci are expressed in mouse kidney. / Field, Loren; Gross, K. W.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, No. 18, 1985, p. 6196-6200.

Research output: Contribution to journalArticle

@article{edc79ecbb42c44d1a741dd8040f630aa,
title = "Ren-1 and Ren-2 loci are expressed in mouse kidney",
abstract = "Inbred strains of mice can be categorized into two groups based on the absence or presence of a duplicated copy of the renin structural gene; one-gene strains carry a single renin gene (Ren-1), whereas two-gene strains carry two renin genes (Ren-1 and Ren-2). To investigate the contribution that each locus makes to the composite levels of renin mRNA observed to accumulate in different tissues of two-gene strains, we have developed two assays capable of distinguishing the highly homologous Ren-1 and Ren-2 transcripts. Both methods take advantage of established base sequence differences between Ren-1 and Ren-2 coding regions by using reverse transcriptase-mediated primer extension of oligonucleotide primer/mRNA hybrids in the presence of appropriate deoxy- and dideoxynucleotide phosphates. Using these techniques we found that Ren-1 and Ren-2 mRNAs accumulate in the kidney of two-gene strains to approximately equal levels. These observations are discussed in light of potential mechanisms regulating the tissue-specific expression of the Ren-1 and Ren-2 loci.",
author = "Loren Field and Gross, {K. W.}",
year = "1985",
language = "English (US)",
volume = "82",
pages = "6196--6200",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "18",

}

TY - JOUR

T1 - Ren-1 and Ren-2 loci are expressed in mouse kidney

AU - Field, Loren

AU - Gross, K. W.

PY - 1985

Y1 - 1985

N2 - Inbred strains of mice can be categorized into two groups based on the absence or presence of a duplicated copy of the renin structural gene; one-gene strains carry a single renin gene (Ren-1), whereas two-gene strains carry two renin genes (Ren-1 and Ren-2). To investigate the contribution that each locus makes to the composite levels of renin mRNA observed to accumulate in different tissues of two-gene strains, we have developed two assays capable of distinguishing the highly homologous Ren-1 and Ren-2 transcripts. Both methods take advantage of established base sequence differences between Ren-1 and Ren-2 coding regions by using reverse transcriptase-mediated primer extension of oligonucleotide primer/mRNA hybrids in the presence of appropriate deoxy- and dideoxynucleotide phosphates. Using these techniques we found that Ren-1 and Ren-2 mRNAs accumulate in the kidney of two-gene strains to approximately equal levels. These observations are discussed in light of potential mechanisms regulating the tissue-specific expression of the Ren-1 and Ren-2 loci.

AB - Inbred strains of mice can be categorized into two groups based on the absence or presence of a duplicated copy of the renin structural gene; one-gene strains carry a single renin gene (Ren-1), whereas two-gene strains carry two renin genes (Ren-1 and Ren-2). To investigate the contribution that each locus makes to the composite levels of renin mRNA observed to accumulate in different tissues of two-gene strains, we have developed two assays capable of distinguishing the highly homologous Ren-1 and Ren-2 transcripts. Both methods take advantage of established base sequence differences between Ren-1 and Ren-2 coding regions by using reverse transcriptase-mediated primer extension of oligonucleotide primer/mRNA hybrids in the presence of appropriate deoxy- and dideoxynucleotide phosphates. Using these techniques we found that Ren-1 and Ren-2 mRNAs accumulate in the kidney of two-gene strains to approximately equal levels. These observations are discussed in light of potential mechanisms regulating the tissue-specific expression of the Ren-1 and Ren-2 loci.

UR - http://www.scopus.com/inward/record.url?scp=0022343497&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022343497&partnerID=8YFLogxK

M3 - Article

VL - 82

SP - 6196

EP - 6200

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 18

ER -