Ren-1 and Ren-2 loci are expressed in mouse kidney

L. J. Field, K. W. Gross

Research output: Contribution to journalArticle

67 Scopus citations

Abstract

Inbred strains of mice can be categorized into two groups based on the absence or presence of a duplicated copy of the renin structural gene; one-gene strains carry a single renin gene (Ren-1), whereas two-gene strains carry two renin genes (Ren-1 and Ren-2). To investigate the contribution that each locus makes to the composite levels of renin mRNA observed to accumulate in different tissues of two-gene strains, we have developed two assays capable of distinguishing the highly homologous Ren-1 and Ren-2 transcripts. Both methods take advantage of established base sequence differences between Ren-1 and Ren-2 coding regions by using reverse transcriptase-mediated primer extension of oligonucleotide primer/mRNA hybrids in the presence of appropriate deoxy- and dideoxynucleotide phosphates. Using these techniques we found that Ren-1 and Ren-2 mRNAs accumulate in the kidney of two-gene strains to approximately equal levels. These observations are discussed in light of potential mechanisms regulating the tissue-specific expression of the Ren-1 and Ren-2 loci.

Original languageEnglish (US)
Pages (from-to)6196-6200
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number18
DOIs
StatePublished - 1985

ASJC Scopus subject areas

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