Renal ischemia induces tropomyosin dissociation-destabilizing microvilli microfilaments

Sharon L. Ashworth, Sarah E. Wean, Silvia B. Campos, Constance J. Temm-Grove, Erica L. Southgate, Bernadette Vrhovski, Peter Gunning, Ron P. Weinberger, Bruce A. Molitoris

Research output: Contribution to journalArticle

18 Scopus citations


Ischemic-induced cell injury results in rapid duration-dependent actin-depolymerizing factor (ADF)/cofilin-mediated disruption of the apical microvilli microfilament cores. Because intestinal microvillar microfilaments are bound and stabilized in the terminal web by the actin-binding protein tropomyosin, we questioned whether a protective effect of tropomyosin localization to the terminal web of the proximal tubule microfilament cores is disrupted during ischemic injury. With tropomyosin-specific antibodies, we examined rat cortical sections under physiological conditions and following ischemic injury by confocal microscopy. In addition, Western blot analysis of cortical extracts and urine was undertaken. Our studies demonstrated the presence of tropomyosin isoforms in the proximal tubule microvillar terminal web under physiological conditions and their dissociation in response to 25 min of ischemic injury. This correlated with the excretion of tropomyosin-containing plasma membrane vesicles in urine from ischemic rats. In addition, we noted increased tropomyosin Triton X-100 solubility following ischemia in cortical extracts. These studies suggest tropomyosin binds to and stabilizes the microvillar microfilament core in the terminal web under physiological conditions. With the onset of ischemic injury, we propose that tropomyosin dissociates from the microfilament core providing access to microfilaments in the terminal web for F-actin binding, severing and depolymerizing actions of ADF/cofilin proteins.

Original languageEnglish (US)
Pages (from-to)F988-F996
JournalAmerican Journal of Physiology - Renal Physiology
Issue number5 55-5
StatePublished - May 1 2004


  • Actin-depolymerizing factor
  • Brush border
  • Cofilin

ASJC Scopus subject areas

  • Physiology

Fingerprint Dive into the research topics of 'Renal ischemia induces tropomyosin dissociation-destabilizing microvilli microfilaments'. Together they form a unique fingerprint.

  • Cite this

    Ashworth, S. L., Wean, S. E., Campos, S. B., Temm-Grove, C. J., Southgate, E. L., Vrhovski, B., Gunning, P., Weinberger, R. P., & Molitoris, B. A. (2004). Renal ischemia induces tropomyosin dissociation-destabilizing microvilli microfilaments. American Journal of Physiology - Renal Physiology, 286(5 55-5), F988-F996.