Repair of 8 oxoguanine in mammalian cells expressing the Drosophila S3 ribosomal/repair protein

Enrico Cappelli, Andrea D'Osualdo, Massimo Bogliolo, Yi Xu, Mark R. Kelley, Guido Frosina

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

8-oxo-7,8-dihydroguanine (8-oxoG) is a potent mutagenic lesion that forms at elevated levels in cellular DNA and is repaired with low efficiency in human cells. Unlike its human counterpart, the Drosophila S3 ribosomal/repair protein is endowed with a vigorous 8 oxoG repair activity that is associated to β,δ-elimination AP lyase activity. We have recently observed that pure GST-tagged Drosophila S3 protein can significantly accelerate the in vitro repair of 8 oxoG performed by human and mouse cell extracts [Cappelli et al., unpublished data]. In this work, we have transfected Chinese hamster cells with mammalian expression vectors containing the Drosophila S3 cDNA. The cells synthesized both S3 mRNA and protein but no improved repair of 8 oxoguanine was observed. Factors important for the proper expression of Drosophila genes in mammalian cells are discussed.

Original languageEnglish (US)
Pages (from-to)113-121
Number of pages9
JournalTeratogenesis Carcinogenesis and Mutagenesis
Issue numberSUPPL. 1
StatePublished - Mar 15 2003

ASJC Scopus subject areas

  • Oncology
  • Genetics
  • Toxicology
  • Genetics(clinical)
  • Health, Toxicology and Mutagenesis

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