Repair of an osseous facial critical-size defect using augmented fibrin sealant

Sunil S. Tholpady, Rodney Schlosser, William Spotnitz, Roy C. Ogle, William H. Lindsey

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Objective: Osseous defects of the head and neck are a common challenge for the otolaryngologist. To develop improved reconstructive options, osteoconductive engineering experiments are being conducted. A nasal critical-size defect (CSD) model has previously been described in which less than 7% bone healing is observed over 6 months. An implant containing fibrin sealant with and without osteoprogenitor cells is evaluated in this model. Study Design: Randomized controlled trial using a rodent model. Methods: A nasal CSD was surgically created in 18 male retired breeder Sprague-Dawley rats. Six animals were not implanted with any material, six received fibrin sealant consisting of fibrin (25 mg/mL) and thrombin (1000 U/mL), and six were implanted with fibrin sealant and rat calvarial osteoprogenitor cells (1.8 x 106 cells/mL). Thirty days later, the animals were examined at necropsy by planimetry, histological analysis of new bone growth, and radiodensitometric analysis of bone thickness. Results: A thin layer of bone covered the defect in all of the treated animals. A statistically significant increase in bone density (P <.05) between fibrin sealant plus osteoprogenitor cells and each of the other groups was shown using radiodensitometric analysis. Histological analysis also confirmed this difference. Conclusion: Osteoprogenitor cells contained within fibrin sealant result in a greater augmentation of bone regeneration than controls or fibrin sealant alone.

Original languageEnglish (US)
Pages (from-to)1585-1588
Number of pages4
JournalLaryngoscope
Volume109
Issue number10
DOIs
StatePublished - Oct 1999
Externally publishedYes

Fingerprint

Fibrin Tissue Adhesive
Nose
Bone and Bones
Bone Regeneration
Bone Development
Fibrin
Thrombin
Bone Density
Sprague Dawley Rats
Rodentia
Neck
Randomized Controlled Trials
Head

Keywords

  • Bone regeneration
  • Critical-size defect
  • Fibrin sealant
  • Nasal reconstruction
  • Tissue engineering

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Tholpady, S. S., Schlosser, R., Spotnitz, W., Ogle, R. C., & Lindsey, W. H. (1999). Repair of an osseous facial critical-size defect using augmented fibrin sealant. Laryngoscope, 109(10), 1585-1588. https://doi.org/10.1097/00005537-199910000-00007

Repair of an osseous facial critical-size defect using augmented fibrin sealant. / Tholpady, Sunil S.; Schlosser, Rodney; Spotnitz, William; Ogle, Roy C.; Lindsey, William H.

In: Laryngoscope, Vol. 109, No. 10, 10.1999, p. 1585-1588.

Research output: Contribution to journalArticle

Tholpady, SS, Schlosser, R, Spotnitz, W, Ogle, RC & Lindsey, WH 1999, 'Repair of an osseous facial critical-size defect using augmented fibrin sealant', Laryngoscope, vol. 109, no. 10, pp. 1585-1588. https://doi.org/10.1097/00005537-199910000-00007
Tholpady, Sunil S. ; Schlosser, Rodney ; Spotnitz, William ; Ogle, Roy C. ; Lindsey, William H. / Repair of an osseous facial critical-size defect using augmented fibrin sealant. In: Laryngoscope. 1999 ; Vol. 109, No. 10. pp. 1585-1588.
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