Repertoire development and ligand specificity of murine TCR gamma delta cells.

J. A. Bluestone, R. Q. Cron, T. A. Barrett, B. Houlden, A. I. Sperling, Alexander Dent, S. Hedrick, B. Rellahan, L. A. Matis

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Abstract

During the past several years, we have been studying the circulating TCR gamma delta cells expressed in peripheral lymphoid tissues. Biochemical and molecular characterization of the TCR gamma delta heterodimers present on these TCR gamma delta cells identified 3 TCR gamma proteins, V gamma 2-C gamma 1, V gamma 1.2-C gamma 2, and V gamma 1.1-C gamma 4. In addition, at least 6 different V delta gene products (V delta 2,4,5,6,V alpha 10, V alpha 11) are expressed in peripheral lymphoid tissue. Nucleotide sequence analysis has revealed a great deal of junctional diversity present among the different V gamma and V delta proteins. Thus, compared to other nonlymphoid tissues (e.g., skin), this population of TCR gamma delta cells appears quite extensive. The development and specificity of TCR gamma delta cells has been pursued by two approaches. First, different TCR gamma delta cells clones were generated which recognize MHC-encoded gene products. One clone recognizes an unconventional TL-encoded antigen, whereas others have been shown to recognize either classical MHC class I or class II antigens. The TCR gamma delta receptor genes have been cloned from the TL-specific TCR gamma delta cell and used to construct transgenic mice to examine the development of TCR gamma delta cells. Although the Tg+ TCR gamma delta cells are tolerized by thymic clonal tolerance similar to TCR alpha beta cells, the epithelial Tg+ TCR gamma delta cells are subjected to non-deletional tolerance (anergy). A second approach towards examining the development of TCR gamma delta cells has been to compare the repertoire of TCR gamma delta splenocytes in a variety of inbred and MHC-congenic strains of mice using subset-specific anti-murine TCR gamma delta mAb. The percentage of individual subsets of splenic TCR gamma delta cells differ widely between different inbred strains of mice due to both MHC- and TCR-encoded genetic differences. In summary, these studies provides a basis for understanding and determining the ligand(s) of the TCR gamma delta heterodimer and the factors which shape the peripheral TCR gamma delta repertoire.

Original languageEnglish (US)
Pages (from-to)5-33
Number of pages29
JournalImmunological Reviews
Volume120
StatePublished - Apr 1991
Externally publishedYes

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Somatostatin-Secreting Cells
Ligands
Lymphoid Tissue
Clone Cells
Genes
Congenic Mice
delta Opioid Receptor
Inbred Strains Mice
Histocompatibility Antigens Class II
Transgenic Mice
Sequence Analysis
Epithelial Cells

ASJC Scopus subject areas

  • Immunology

Cite this

Bluestone, J. A., Cron, R. Q., Barrett, T. A., Houlden, B., Sperling, A. I., Dent, A., ... Matis, L. A. (1991). Repertoire development and ligand specificity of murine TCR gamma delta cells. Immunological Reviews, 120, 5-33.

Repertoire development and ligand specificity of murine TCR gamma delta cells. / Bluestone, J. A.; Cron, R. Q.; Barrett, T. A.; Houlden, B.; Sperling, A. I.; Dent, Alexander; Hedrick, S.; Rellahan, B.; Matis, L. A.

In: Immunological Reviews, Vol. 120, 04.1991, p. 5-33.

Research output: Contribution to journalArticle

Bluestone, JA, Cron, RQ, Barrett, TA, Houlden, B, Sperling, AI, Dent, A, Hedrick, S, Rellahan, B & Matis, LA 1991, 'Repertoire development and ligand specificity of murine TCR gamma delta cells.', Immunological Reviews, vol. 120, pp. 5-33.
Bluestone JA, Cron RQ, Barrett TA, Houlden B, Sperling AI, Dent A et al. Repertoire development and ligand specificity of murine TCR gamma delta cells. Immunological Reviews. 1991 Apr;120:5-33.
Bluestone, J. A. ; Cron, R. Q. ; Barrett, T. A. ; Houlden, B. ; Sperling, A. I. ; Dent, Alexander ; Hedrick, S. ; Rellahan, B. ; Matis, L. A. / Repertoire development and ligand specificity of murine TCR gamma delta cells. In: Immunological Reviews. 1991 ; Vol. 120. pp. 5-33.
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abstract = "During the past several years, we have been studying the circulating TCR gamma delta cells expressed in peripheral lymphoid tissues. Biochemical and molecular characterization of the TCR gamma delta heterodimers present on these TCR gamma delta cells identified 3 TCR gamma proteins, V gamma 2-C gamma 1, V gamma 1.2-C gamma 2, and V gamma 1.1-C gamma 4. In addition, at least 6 different V delta gene products (V delta 2,4,5,6,V alpha 10, V alpha 11) are expressed in peripheral lymphoid tissue. Nucleotide sequence analysis has revealed a great deal of junctional diversity present among the different V gamma and V delta proteins. Thus, compared to other nonlymphoid tissues (e.g., skin), this population of TCR gamma delta cells appears quite extensive. The development and specificity of TCR gamma delta cells has been pursued by two approaches. First, different TCR gamma delta cells clones were generated which recognize MHC-encoded gene products. One clone recognizes an unconventional TL-encoded antigen, whereas others have been shown to recognize either classical MHC class I or class II antigens. The TCR gamma delta receptor genes have been cloned from the TL-specific TCR gamma delta cell and used to construct transgenic mice to examine the development of TCR gamma delta cells. Although the Tg+ TCR gamma delta cells are tolerized by thymic clonal tolerance similar to TCR alpha beta cells, the epithelial Tg+ TCR gamma delta cells are subjected to non-deletional tolerance (anergy). A second approach towards examining the development of TCR gamma delta cells has been to compare the repertoire of TCR gamma delta splenocytes in a variety of inbred and MHC-congenic strains of mice using subset-specific anti-murine TCR gamma delta mAb. The percentage of individual subsets of splenic TCR gamma delta cells differ widely between different inbred strains of mice due to both MHC- and TCR-encoded genetic differences. In summary, these studies provides a basis for understanding and determining the ligand(s) of the TCR gamma delta heterodimer and the factors which shape the peripheral TCR gamma delta repertoire.",
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AU - Cron, R. Q.

AU - Barrett, T. A.

AU - Houlden, B.

AU - Sperling, A. I.

AU - Dent, Alexander

AU - Hedrick, S.

AU - Rellahan, B.

AU - Matis, L. A.

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N2 - During the past several years, we have been studying the circulating TCR gamma delta cells expressed in peripheral lymphoid tissues. Biochemical and molecular characterization of the TCR gamma delta heterodimers present on these TCR gamma delta cells identified 3 TCR gamma proteins, V gamma 2-C gamma 1, V gamma 1.2-C gamma 2, and V gamma 1.1-C gamma 4. In addition, at least 6 different V delta gene products (V delta 2,4,5,6,V alpha 10, V alpha 11) are expressed in peripheral lymphoid tissue. Nucleotide sequence analysis has revealed a great deal of junctional diversity present among the different V gamma and V delta proteins. Thus, compared to other nonlymphoid tissues (e.g., skin), this population of TCR gamma delta cells appears quite extensive. The development and specificity of TCR gamma delta cells has been pursued by two approaches. First, different TCR gamma delta cells clones were generated which recognize MHC-encoded gene products. One clone recognizes an unconventional TL-encoded antigen, whereas others have been shown to recognize either classical MHC class I or class II antigens. The TCR gamma delta receptor genes have been cloned from the TL-specific TCR gamma delta cell and used to construct transgenic mice to examine the development of TCR gamma delta cells. Although the Tg+ TCR gamma delta cells are tolerized by thymic clonal tolerance similar to TCR alpha beta cells, the epithelial Tg+ TCR gamma delta cells are subjected to non-deletional tolerance (anergy). A second approach towards examining the development of TCR gamma delta cells has been to compare the repertoire of TCR gamma delta splenocytes in a variety of inbred and MHC-congenic strains of mice using subset-specific anti-murine TCR gamma delta mAb. The percentage of individual subsets of splenic TCR gamma delta cells differ widely between different inbred strains of mice due to both MHC- and TCR-encoded genetic differences. In summary, these studies provides a basis for understanding and determining the ligand(s) of the TCR gamma delta heterodimer and the factors which shape the peripheral TCR gamma delta repertoire.

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