Repigmentation of human retinal pigment epithelial cells in vitro

Mike Boulton, John Marshall

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

Cultured human retinal pigment epithelial (RPE) cells readily ingested both melanin and lipofuscin isolated from human RPE cells. Up to 7 days post-challenge ingested granules demonstrated no evidence of lysis or aggregation within secondary lysosomes. When cultures containing ingested melanin and lipofuscin were subcultured the cells gradually depigmented due to a redistribution of pigment granules amongst daughter cells. Quantitative analysis demonstrated that the accumulation of both types of granule was linear over a 24 hr challenge period. This study reports a technique of 'artificially' repigmenting cultured human RPE cells and thus offers the potential for in vitro investigations of the role of these inclusions in various dynamic aspects of cellular metabolism.

Original languageEnglish (US)
Pages (from-to)209-218
Number of pages10
JournalExperimental eye research
Volume41
Issue number2
DOIs
StatePublished - Aug 1985

Keywords

  • lipofuscin
  • melanin
  • phagocytosis
  • repigmentation
  • retinal pigment epithelium
  • tissue culture

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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