Replication of the 4p16 susceptibility locus in congenital heart disease in han Chinese populations

Bijun Zhao, Yuan Lin, Jing Xu, Bixian Ni, Min Da, Chenyue Ding, Yuanli Hu, Kai Zhang, Shiwei Yang, Xiaowei Wang, Shiqiang Yu, Yijiang Chen, Xuming Mo, Jiayin Liu, Hongbing Shen, Jiahao Sha, Hongxia Ma

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Abstract

Congenital heart disease (CHD) is the most common form of congenital human birth anomalies and a leading cause of perinatal and infant mortality. Some studies including our published genome-wide association study (GWAS) of CHD have indicated that genetic variants may contribute to the risk of CHD. Recently, Cordell et al. published a GWAS of multiple CHD phenotypes in European Caucasians and identified 3 susceptibility loci (rs870142, rs16835979 and rs6824295) for ostium secundum atrial septal defect (ASD) at chromosome 4p16. However, whether these loci at 4p16 confer the predisposition to CHD in Chinese population is unclear. In the current study, we first analyzed the associations between these 3 single nucleotide polymorphisms (SNPs) at 4p16 and CHD risk by using our existing genome-wide scan data and found all of the 3 SNPs showed significant associations with ASD in the same direction as that observed in Cordell's study, but not with other subtypes- ventricular septal defect (VSD) and ASD combined VSD. As these 3 SNPs were in high linkage disequilibrium (LD) in Chinese population, we selected one SNP with the lowest P value in our GWAS scan (rs16835979) to perform a replication study with additional 1,709 CHD cases with multiple phenotypes and 1,962 controls. The significant association was also observed only within the ASD subgroup, which was heterogeneous from other disease groups. In combined GWAS and replication samples, the minor allele of rs16835979 remained significant association with the risk of ASD (OR = 1.22, 95% CI = 1.08-1.38, P = 0.001). Our findings suggest that susceptibility loci of ASD identified from Cordell's European GWAS are generalizable to Chinese population, and such investigation may provide new insights into the roles of genetic variants in the etiology of different CHD phenotypes.

Original languageEnglish (US)
Article numbere0107411
JournalPLoS One
Volume9
Issue number9
DOIs
StatePublished - Sep 1 2014
Externally publishedYes

Fingerprint

heart diseases
Atrial Heart Septal Defects
Genome-Wide Association Study
Heart Diseases
loci
Defects
Genes
single nucleotide polymorphism
Population
Polymorphism
Single Nucleotide Polymorphism
Nucleotides
Ventricular Heart Septal Defects
Phenotype
phenotype
infant mortality
Perinatal Mortality
Linkage Disequilibrium
Infant Mortality
linkage disequilibrium

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Replication of the 4p16 susceptibility locus in congenital heart disease in han Chinese populations. / Zhao, Bijun; Lin, Yuan; Xu, Jing; Ni, Bixian; Da, Min; Ding, Chenyue; Hu, Yuanli; Zhang, Kai; Yang, Shiwei; Wang, Xiaowei; Yu, Shiqiang; Chen, Yijiang; Mo, Xuming; Liu, Jiayin; Shen, Hongbing; Sha, Jiahao; Ma, Hongxia.

In: PLoS One, Vol. 9, No. 9, e0107411, 01.09.2014.

Research output: Contribution to journalArticle

Zhao, B, Lin, Y, Xu, J, Ni, B, Da, M, Ding, C, Hu, Y, Zhang, K, Yang, S, Wang, X, Yu, S, Chen, Y, Mo, X, Liu, J, Shen, H, Sha, J & Ma, H 2014, 'Replication of the 4p16 susceptibility locus in congenital heart disease in han Chinese populations', PLoS One, vol. 9, no. 9, e0107411. https://doi.org/10.1371/journal.pone.0107411
Zhao, Bijun ; Lin, Yuan ; Xu, Jing ; Ni, Bixian ; Da, Min ; Ding, Chenyue ; Hu, Yuanli ; Zhang, Kai ; Yang, Shiwei ; Wang, Xiaowei ; Yu, Shiqiang ; Chen, Yijiang ; Mo, Xuming ; Liu, Jiayin ; Shen, Hongbing ; Sha, Jiahao ; Ma, Hongxia. / Replication of the 4p16 susceptibility locus in congenital heart disease in han Chinese populations. In: PLoS One. 2014 ; Vol. 9, No. 9.
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abstract = "Congenital heart disease (CHD) is the most common form of congenital human birth anomalies and a leading cause of perinatal and infant mortality. Some studies including our published genome-wide association study (GWAS) of CHD have indicated that genetic variants may contribute to the risk of CHD. Recently, Cordell et al. published a GWAS of multiple CHD phenotypes in European Caucasians and identified 3 susceptibility loci (rs870142, rs16835979 and rs6824295) for ostium secundum atrial septal defect (ASD) at chromosome 4p16. However, whether these loci at 4p16 confer the predisposition to CHD in Chinese population is unclear. In the current study, we first analyzed the associations between these 3 single nucleotide polymorphisms (SNPs) at 4p16 and CHD risk by using our existing genome-wide scan data and found all of the 3 SNPs showed significant associations with ASD in the same direction as that observed in Cordell's study, but not with other subtypes- ventricular septal defect (VSD) and ASD combined VSD. As these 3 SNPs were in high linkage disequilibrium (LD) in Chinese population, we selected one SNP with the lowest P value in our GWAS scan (rs16835979) to perform a replication study with additional 1,709 CHD cases with multiple phenotypes and 1,962 controls. The significant association was also observed only within the ASD subgroup, which was heterogeneous from other disease groups. In combined GWAS and replication samples, the minor allele of rs16835979 remained significant association with the risk of ASD (OR = 1.22, 95{\%} CI = 1.08-1.38, P = 0.001). Our findings suggest that susceptibility loci of ASD identified from Cordell's European GWAS are generalizable to Chinese population, and such investigation may provide new insights into the roles of genetic variants in the etiology of different CHD phenotypes.",
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AU - Zhang, Kai

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