Replication of the association between the thrombospondin-4 A387P polymorphism and myocardial infarction

Jennifer Wessel, Eric J. Topol, Ming Ji, Joanne Meyer, Jeanette J. McCarthy

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Background The purpose of our study was to replicate an association between the A387P polymorphism in the thrombospondin-4 (THBS4) gene and myocardial infarction (MI), as previously reported by our group while taking confounding into account, and to assess whether ascertainment by age of onset would affect this association. Methods We performed a case-control study of 474 white patients with MI (not selected on the basis of age of onset nor family history) and 472 white control subjects. We then applied our findings to our original population of 184 white patients with premature, familial MI and 406 white control subjects. Results In the replication population, no significant association was found between THBS4 genotype and MI (P = .41) with univariate analysis. However, after adjusting for age, sex, first-degree family history, and waist-to-hip ratio, an association was apparent in the replication population (P = .032), and the original association became much stronger (P = .00008). Both studies showed a 2.5- to 3-fold increased odds of MI in individuals with the P allele. Furthermore, several variables appeared to modify the effect of THBS4 on MI, including waist-to-hip ratio, diabetes mellitus, and hypertension. When we stratified our cases by age of onset (≤45 years in men, ≤50 years in women), there were no significant differences in genotype frequencies when comparing premature cases with late-onset cases or premature cases with control subjects in either unadjusted or adjusted analyses (all P values >.25). Conclusions Our findings suggest that the A387P variant of the THBS4 gene may be an important determinant in the development of MI at any age. Careful assessment of clinical covariates helped to unmask a significant association and therefore may be an important reason for why studies do not replicate.

Original languageEnglish (US)
Pages (from-to)905-909
Number of pages5
JournalAmerican Heart Journal
Volume147
Issue number5
DOIs
StatePublished - May 2004
Externally publishedYes

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Myocardial Infarction
Age of Onset
Waist-Hip Ratio
Genotype
Population
thrombospondin 4
Genes
Case-Control Studies
Diabetes Mellitus
Alleles
Hypertension

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Replication of the association between the thrombospondin-4 A387P polymorphism and myocardial infarction. / Wessel, Jennifer; Topol, Eric J.; Ji, Ming; Meyer, Joanne; McCarthy, Jeanette J.

In: American Heart Journal, Vol. 147, No. 5, 05.2004, p. 905-909.

Research output: Contribution to journalArticle

Wessel, Jennifer ; Topol, Eric J. ; Ji, Ming ; Meyer, Joanne ; McCarthy, Jeanette J. / Replication of the association between the thrombospondin-4 A387P polymorphism and myocardial infarction. In: American Heart Journal. 2004 ; Vol. 147, No. 5. pp. 905-909.
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AB - Background The purpose of our study was to replicate an association between the A387P polymorphism in the thrombospondin-4 (THBS4) gene and myocardial infarction (MI), as previously reported by our group while taking confounding into account, and to assess whether ascertainment by age of onset would affect this association. Methods We performed a case-control study of 474 white patients with MI (not selected on the basis of age of onset nor family history) and 472 white control subjects. We then applied our findings to our original population of 184 white patients with premature, familial MI and 406 white control subjects. Results In the replication population, no significant association was found between THBS4 genotype and MI (P = .41) with univariate analysis. However, after adjusting for age, sex, first-degree family history, and waist-to-hip ratio, an association was apparent in the replication population (P = .032), and the original association became much stronger (P = .00008). Both studies showed a 2.5- to 3-fold increased odds of MI in individuals with the P allele. Furthermore, several variables appeared to modify the effect of THBS4 on MI, including waist-to-hip ratio, diabetes mellitus, and hypertension. When we stratified our cases by age of onset (≤45 years in men, ≤50 years in women), there were no significant differences in genotype frequencies when comparing premature cases with late-onset cases or premature cases with control subjects in either unadjusted or adjusted analyses (all P values >.25). Conclusions Our findings suggest that the A387P variant of the THBS4 gene may be an important determinant in the development of MI at any age. Careful assessment of clinical covariates helped to unmask a significant association and therefore may be an important reason for why studies do not replicate.

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