Repression of transcription by Rgt1 in the absence of glucose requires Std1 and Mth1

Jaganathan Lakshmanan, Amber Mosley, Sabire Özcan

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

In the yeast Saccharomyces cerevisiae, glucose induces expression of the hexose transporter (HXT) genes by inhibiting the repressor function of the transcription factor Rgt1. We have previously shown that Rgt1 binds to the HXT gene promoters only in the absence of glucose. In the presence of glucose, Rgt1 becomes phosphorylated and is unable to bind to the HXT promoters and repress their transcription. We report that Rgt1 interacts with Std1 and Mth1 in a yeast two-hybrid assay and co-immunoprecipitates with both proteins in vivo only when glucose is absent. In addition, we demonstrate that repression of HXT gene expression by Rgt1 is abolished in the std1 mth1 double mutant. While Rgt1 is normally phosphorylated only in the presence of high concentrations of glucose, it is constitutively modified in the std1 mth1 double mutant. Based on these data, we conclude that, in the absence of glucose, Rgt1 associates with Std1 and Mth1 to repress HXT gene expression.

Original languageEnglish (US)
Pages (from-to)19-25
Number of pages7
JournalCurrent Genetics
Volume44
Issue number1
DOIs
StatePublished - Oct 1 2003
Externally publishedYes

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Monosaccharide Transport Proteins
Glucose
Gene Expression
Two-Hybrid System Techniques
Genes
Saccharomyces cerevisiae
Transcription Factors
Yeasts
Proteins

Keywords

  • Glucose
  • HXT
  • Mth1
  • Rgt1
  • Std1
  • Yeast

ASJC Scopus subject areas

  • Genetics

Cite this

Repression of transcription by Rgt1 in the absence of glucose requires Std1 and Mth1. / Lakshmanan, Jaganathan; Mosley, Amber; Özcan, Sabire.

In: Current Genetics, Vol. 44, No. 1, 01.10.2003, p. 19-25.

Research output: Contribution to journalArticle

Lakshmanan, Jaganathan ; Mosley, Amber ; Özcan, Sabire. / Repression of transcription by Rgt1 in the absence of glucose requires Std1 and Mth1. In: Current Genetics. 2003 ; Vol. 44, No. 1. pp. 19-25.
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