Reproducibility of circadian retinal and optic nerve head blood flow measurements by Heidelberg retina flowmetry

C. P. Jonescu-Cuypers, Alon Harris, K. U. Bartz-Schmidt, L. Kagemann, A. S. Boros, U. E. Heimann, B. H. Lenz, R. D. Hilgers, G. K. Krieglstein

Research output: Contribution to journalArticle

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Abstract

Background/aim: The Heidelberg retina flowmeter (HRF) is designed to measure retinal capillary blood flow. Previous studies however showed weak reproducibility of data. The intraindividual reproducibility of circadian HRF measurements was examined in healthy subjects in three locations of the retina. Methods: 36 healthy volunteers (27.3 (SD 4.3) years) were examined by HRF seven times a day (t0-t6). Using a default window of 10x10 pixels, three consecutive measurements were performed in three precise focusing planes: superficial, intermediate and deep layer, peripapillary retina, neuroretinal rim and cup, respectively. Images of identical tissue locations identified by capillary landmarks of each layer were selected to quantify the retinal microcirculation of each volunteer. Means and standard deviations of all flow results of a given subject were calculated, at t0-t6 and the coefficients of variation as a measure of reproducibility. Results: The coefficients of variation ranged between 8.4% and 41.0% in the superficial layer (mean 19.8% (SD 8.4%)), 10.6%, and 43.0% in the intermediate layer (mean 24.0% (SD 8.4%)), and 9.9% and 84.0% (mean 29.6% (SD 15.8%)) in the deep layer. Conclusions: These data show the best reproducibility of measurements in the superficial layer followed by the intermediate and the deep layer. Clinically, this is an unsatisfactory intraindividual reproducibility of flow values in each studied layer.

Original languageEnglish
Pages (from-to)348-353
Number of pages6
JournalBritish Journal of Ophthalmology
Volume88
Issue number3
DOIs
StatePublished - Mar 2004

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Rheology
Optic Disk
Retina
Flowmeters
Healthy Volunteers
Microcirculation
Volunteers

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Jonescu-Cuypers, C. P., Harris, A., Bartz-Schmidt, K. U., Kagemann, L., Boros, A. S., Heimann, U. E., ... Krieglstein, G. K. (2004). Reproducibility of circadian retinal and optic nerve head blood flow measurements by Heidelberg retina flowmetry. British Journal of Ophthalmology, 88(3), 348-353. https://doi.org/10.1136/bjo.2003.024885

Reproducibility of circadian retinal and optic nerve head blood flow measurements by Heidelberg retina flowmetry. / Jonescu-Cuypers, C. P.; Harris, Alon; Bartz-Schmidt, K. U.; Kagemann, L.; Boros, A. S.; Heimann, U. E.; Lenz, B. H.; Hilgers, R. D.; Krieglstein, G. K.

In: British Journal of Ophthalmology, Vol. 88, No. 3, 03.2004, p. 348-353.

Research output: Contribution to journalArticle

Jonescu-Cuypers, CP, Harris, A, Bartz-Schmidt, KU, Kagemann, L, Boros, AS, Heimann, UE, Lenz, BH, Hilgers, RD & Krieglstein, GK 2004, 'Reproducibility of circadian retinal and optic nerve head blood flow measurements by Heidelberg retina flowmetry', British Journal of Ophthalmology, vol. 88, no. 3, pp. 348-353. https://doi.org/10.1136/bjo.2003.024885
Jonescu-Cuypers, C. P. ; Harris, Alon ; Bartz-Schmidt, K. U. ; Kagemann, L. ; Boros, A. S. ; Heimann, U. E. ; Lenz, B. H. ; Hilgers, R. D. ; Krieglstein, G. K. / Reproducibility of circadian retinal and optic nerve head blood flow measurements by Heidelberg retina flowmetry. In: British Journal of Ophthalmology. 2004 ; Vol. 88, No. 3. pp. 348-353.
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abstract = "Background/aim: The Heidelberg retina flowmeter (HRF) is designed to measure retinal capillary blood flow. Previous studies however showed weak reproducibility of data. The intraindividual reproducibility of circadian HRF measurements was examined in healthy subjects in three locations of the retina. Methods: 36 healthy volunteers (27.3 (SD 4.3) years) were examined by HRF seven times a day (t0-t6). Using a default window of 10x10 pixels, three consecutive measurements were performed in three precise focusing planes: superficial, intermediate and deep layer, peripapillary retina, neuroretinal rim and cup, respectively. Images of identical tissue locations identified by capillary landmarks of each layer were selected to quantify the retinal microcirculation of each volunteer. Means and standard deviations of all flow results of a given subject were calculated, at t0-t6 and the coefficients of variation as a measure of reproducibility. Results: The coefficients of variation ranged between 8.4{\%} and 41.0{\%} in the superficial layer (mean 19.8{\%} (SD 8.4{\%})), 10.6{\%}, and 43.0{\%} in the intermediate layer (mean 24.0{\%} (SD 8.4{\%})), and 9.9{\%} and 84.0{\%} (mean 29.6{\%} (SD 15.8{\%})) in the deep layer. Conclusions: These data show the best reproducibility of measurements in the superficial layer followed by the intermediate and the deep layer. Clinically, this is an unsatisfactory intraindividual reproducibility of flow values in each studied layer.",
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AU - Boros, A. S.

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