Reproducibility of the Heidelberg retinal flowmeter in determining low perfusion areas in peripapillary retina

C. P. Jonescu-Cuypers, Alon Harris, R. Wilson, L. Kagemann, L. V. Mavroudis, F. Topouzis, A. L. Coleman

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Abstract

Aim: To evaluate the interobserver variability and retest reproducibility of confocal scanning laser Doppler flowmeter in measuring capillary perfusion of the peripapillary retina. Methods: Blood flow measurements were performed in one eye of 10 normal subjects by two investigators on two different days (visits). Five separate measurements of the peripapillary blood flow parameters were recorded by each observer at each visit. The Heidelberg retina flowmeter was used to record capillary perfusion in a 2560×640 μm area of the superotemporal peripapillary region and pixel by pixel analysis was done from an area adjacent to the optic disc, with a minimum of 1600 pixels. The percentage of pixels with less than 1 arbitrary unit of flow (no flow) and 10, 25, 50, 75, 90th percentiles of flow values was calculated. Interobserver measurements were compared by paired t test, Intraclass correlations (ICC) were used to determine the interobserver variability and retest reproducibility of the measurements. Intrasession coefficients of variations (CV) were also calculated. Results: There were no statistically significant differences between the two observers for all measurements and between visits for the percentage of pixels with no flow. ICC was 66% (range 57.09%-77.1%) for pixels with no flow. For the 10, 25, 50, 75, 90th percentiles of flow the ICC was 63.07% (53.91%-77.81%), 71.3% (64.23%-80.85%), 72.61% (66.02%-78.96%), 65.86% (58.53%-74.77%), and 60.05% (54.34%-70.06%), respectively. CV was 16.59%, 11.47%, 9.32%, 9.03%, 11.58%, and 16.05% for the percentage of no flow pixels and the 10, 25,50,75,90th percentiles of flow, respectively. Conclusions: The Heidelberg retinal flowmeter allows reproducible measurements of all levels of capillary perfusion areas when pixel by pixel analysis is used.

Original languageEnglish
Pages (from-to)1266-1269
Number of pages4
JournalBritish Journal of Ophthalmology
Volume88
Issue number10
DOIs
StatePublished - Oct 2004

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Flowmeters
Retina
Observer Variation
Perfusion
Optic Disk
Lasers
Research Personnel

ASJC Scopus subject areas

  • Ophthalmology

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Jonescu-Cuypers, C. P., Harris, A., Wilson, R., Kagemann, L., Mavroudis, L. V., Topouzis, F., & Coleman, A. L. (2004). Reproducibility of the Heidelberg retinal flowmeter in determining low perfusion areas in peripapillary retina. British Journal of Ophthalmology, 88(10), 1266-1269. https://doi.org/10.1136/bjo.2003.039099

Reproducibility of the Heidelberg retinal flowmeter in determining low perfusion areas in peripapillary retina. / Jonescu-Cuypers, C. P.; Harris, Alon; Wilson, R.; Kagemann, L.; Mavroudis, L. V.; Topouzis, F.; Coleman, A. L.

In: British Journal of Ophthalmology, Vol. 88, No. 10, 10.2004, p. 1266-1269.

Research output: Contribution to journalArticle

Jonescu-Cuypers, CP, Harris, A, Wilson, R, Kagemann, L, Mavroudis, LV, Topouzis, F & Coleman, AL 2004, 'Reproducibility of the Heidelberg retinal flowmeter in determining low perfusion areas in peripapillary retina', British Journal of Ophthalmology, vol. 88, no. 10, pp. 1266-1269. https://doi.org/10.1136/bjo.2003.039099
Jonescu-Cuypers, C. P. ; Harris, Alon ; Wilson, R. ; Kagemann, L. ; Mavroudis, L. V. ; Topouzis, F. ; Coleman, A. L. / Reproducibility of the Heidelberg retinal flowmeter in determining low perfusion areas in peripapillary retina. In: British Journal of Ophthalmology. 2004 ; Vol. 88, No. 10. pp. 1266-1269.
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abstract = "Aim: To evaluate the interobserver variability and retest reproducibility of confocal scanning laser Doppler flowmeter in measuring capillary perfusion of the peripapillary retina. Methods: Blood flow measurements were performed in one eye of 10 normal subjects by two investigators on two different days (visits). Five separate measurements of the peripapillary blood flow parameters were recorded by each observer at each visit. The Heidelberg retina flowmeter was used to record capillary perfusion in a 2560×640 μm area of the superotemporal peripapillary region and pixel by pixel analysis was done from an area adjacent to the optic disc, with a minimum of 1600 pixels. The percentage of pixels with less than 1 arbitrary unit of flow (no flow) and 10, 25, 50, 75, 90th percentiles of flow values was calculated. Interobserver measurements were compared by paired t test, Intraclass correlations (ICC) were used to determine the interobserver variability and retest reproducibility of the measurements. Intrasession coefficients of variations (CV) were also calculated. Results: There were no statistically significant differences between the two observers for all measurements and between visits for the percentage of pixels with no flow. ICC was 66{\%} (range 57.09{\%}-77.1{\%}) for pixels with no flow. For the 10, 25, 50, 75, 90th percentiles of flow the ICC was 63.07{\%} (53.91{\%}-77.81{\%}), 71.3{\%} (64.23{\%}-80.85{\%}), 72.61{\%} (66.02{\%}-78.96{\%}), 65.86{\%} (58.53{\%}-74.77{\%}), and 60.05{\%} (54.34{\%}-70.06{\%}), respectively. CV was 16.59{\%}, 11.47{\%}, 9.32{\%}, 9.03{\%}, 11.58{\%}, and 16.05{\%} for the percentage of no flow pixels and the 10, 25,50,75,90th percentiles of flow, respectively. Conclusions: The Heidelberg retinal flowmeter allows reproducible measurements of all levels of capillary perfusion areas when pixel by pixel analysis is used.",
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AU - Wilson, R.

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AU - Mavroudis, L. V.

AU - Topouzis, F.

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