Repulsive Wnt signaling inhibits axon regeneration after CNS injury

Yaobo Liu, Xiaofei Wang, Chin Chun Lu, Rachel Sherman-Kermen, Oswald Steward, Xiao-Ming Xu, Yimin Zou

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Failure of axon regeneration in the mammalian CNS is attributable in part to the presence of various inhibitory molecules, including myelin-associated proteins and proteoglycans enriched in glial scars. Here, we evaluate whether axon guidance molecules also regulate regenerative growth after injury in adulthood. Wnts are a large family of axon guidance molecules that can attract ascending axons and repel descending axons along the length of the developing spinal cord. Their expression (all 19 Wnts) is not detectable in normal adult spinal cord by in situ hybridization. However, three of them are clearly reinduced after spinal cord injury. Wnt1 and Wnt5a, encoding potent repellents of the descending corticospinal tract (CST) axons, were robustly and acutely induced broadly in the spinal cord gray matter after unilateral hemisection. Ryk, the conserved repulsive Wnt receptor, was also induced in the lesion area, and Ryk immunoreactivity was found on the lesioned CST axons. Wnt4, which attracts ascending sensory axons in development, was acutely induced in areas closer to the lesion than Wnt1 and Wnt5a. Injection of function-blocking Ryk antibodies into the dorsal bilateral hemisectioned spinal cord either prevented the retraction of CST axons or promoted their regrowth but clearly enhanced the sprouting of CST collateral branches around and beyond the injury site. Therefore, repulsive Wnt signaling may be a cause of cortical spinal tract axon retraction and inhibits axon sprouting after injury.

Original languageEnglish
Pages (from-to)8376-8382
Number of pages7
JournalJournal of Neuroscience
Volume28
Issue number33
DOIs
StatePublished - Aug 13 2008

Fingerprint

Axons
Regeneration
Wounds and Injuries
Pyramidal Tracts
Spinal Cord
Wnt Receptors
Myelin Proteins
Blocking Antibodies
Proteoglycans
Spinal Cord Injuries
Neuroglia
Cicatrix
In Situ Hybridization
Injections
Growth

Keywords

  • Axon retraction
  • Collateral branches
  • Corticospinal tract
  • Paralysis
  • Ryk
  • Spinal cord injury
  • Wnt1
  • Wnt5a

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Liu, Y., Wang, X., Lu, C. C., Sherman-Kermen, R., Steward, O., Xu, X-M., & Zou, Y. (2008). Repulsive Wnt signaling inhibits axon regeneration after CNS injury. Journal of Neuroscience, 28(33), 8376-8382. https://doi.org/10.1523/JNEUROSCI.1939-08.2008

Repulsive Wnt signaling inhibits axon regeneration after CNS injury. / Liu, Yaobo; Wang, Xiaofei; Lu, Chin Chun; Sherman-Kermen, Rachel; Steward, Oswald; Xu, Xiao-Ming; Zou, Yimin.

In: Journal of Neuroscience, Vol. 28, No. 33, 13.08.2008, p. 8376-8382.

Research output: Contribution to journalArticle

Liu, Y, Wang, X, Lu, CC, Sherman-Kermen, R, Steward, O, Xu, X-M & Zou, Y 2008, 'Repulsive Wnt signaling inhibits axon regeneration after CNS injury', Journal of Neuroscience, vol. 28, no. 33, pp. 8376-8382. https://doi.org/10.1523/JNEUROSCI.1939-08.2008
Liu, Yaobo ; Wang, Xiaofei ; Lu, Chin Chun ; Sherman-Kermen, Rachel ; Steward, Oswald ; Xu, Xiao-Ming ; Zou, Yimin. / Repulsive Wnt signaling inhibits axon regeneration after CNS injury. In: Journal of Neuroscience. 2008 ; Vol. 28, No. 33. pp. 8376-8382.
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