Requirement for mast cell growth factor for primordial germ cell survival in culture

Susanna Dolci, Douglas E. Williams, Mary K. Ernst, James L. Resnick, Camilynn I. Brannan, Leslie F. Lock, Stewart D. Lyman, H. Boswell, Peter J. Donovan

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Abstract

MAST-CELL growth factor (MGF) is encoded by the murine steel (Sl) locus and is a ligand for the tyrosine kinase receptor protein encoded by the proto-oncogene c-kit at the murine dominant white spotting (W) locus. Mutations at both these loci affect mast cells, primordial germ cells (PGCs), haemopoietic stem cells and melanocytes. In many Sl and W mutants, the rapid proliferation of PGC that normally occurs between day 7 and 13.5 of embryonic development fails to occur. As c-kit is expressed in PGCs1,2 while MGF is expressed in the surrounding mesenchyme2,3, MGF might promote the proliferation of PGCs. Here we report that MGF is essential for PGC survival in culture, but does not stimulate PGC proliferation. Moreover, whereas both the transmembrane and soluble proteolytic cleavage forms of MGF stimulate mast-cell proliferation, soluble MGF has a relatively limited ability to support survival of PGCs in culture, thus explaining the sterility in mice carrying the steel-dickie (Sld) mutation, which encodes only a soluble form of MGF, and providing a functional role for a transmembrane growth factor.

Original languageEnglish
Pages (from-to)809-811
Number of pages3
JournalNature
Volume352
Issue number6338
StatePublished - Aug 29 1991

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Stem Cell Factor
Germ Cells
Cell Survival
Intercellular Signaling Peptides and Proteins
Steel
Cell Proliferation
Proto-Oncogene Proteins c-kit
Metrorrhagia
Mutation
Melanocytes
Receptor Protein-Tyrosine Kinases
Mast Cells
Infertility
Embryonic Development
Stem Cells
Cell Culture Techniques
Ligands

ASJC Scopus subject areas

  • General

Cite this

Dolci, S., Williams, D. E., Ernst, M. K., Resnick, J. L., Brannan, C. I., Lock, L. F., ... Donovan, P. J. (1991). Requirement for mast cell growth factor for primordial germ cell survival in culture. Nature, 352(6338), 809-811.

Requirement for mast cell growth factor for primordial germ cell survival in culture. / Dolci, Susanna; Williams, Douglas E.; Ernst, Mary K.; Resnick, James L.; Brannan, Camilynn I.; Lock, Leslie F.; Lyman, Stewart D.; Boswell, H.; Donovan, Peter J.

In: Nature, Vol. 352, No. 6338, 29.08.1991, p. 809-811.

Research output: Contribution to journalArticle

Dolci, S, Williams, DE, Ernst, MK, Resnick, JL, Brannan, CI, Lock, LF, Lyman, SD, Boswell, H & Donovan, PJ 1991, 'Requirement for mast cell growth factor for primordial germ cell survival in culture', Nature, vol. 352, no. 6338, pp. 809-811.
Dolci S, Williams DE, Ernst MK, Resnick JL, Brannan CI, Lock LF et al. Requirement for mast cell growth factor for primordial germ cell survival in culture. Nature. 1991 Aug 29;352(6338):809-811.
Dolci, Susanna ; Williams, Douglas E. ; Ernst, Mary K. ; Resnick, James L. ; Brannan, Camilynn I. ; Lock, Leslie F. ; Lyman, Stewart D. ; Boswell, H. ; Donovan, Peter J. / Requirement for mast cell growth factor for primordial germ cell survival in culture. In: Nature. 1991 ; Vol. 352, No. 6338. pp. 809-811.
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