Requirement for p85α regulatory subunit of class IA PI3K in myeloproliferative disease driven by an activation loop mutant of KIT

Veerendra Munugalavadla, Emily C. Sims, Rebecca J. Chan, Stephen D. Lenz, Reuben Kapur

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Objective: Oncogenic activation loop mutations of KIT are observed in acute myeloid leukemia (AML) and in myeloproliferative disorders (MPD); however, the signaling pathways that contribute to transformation via these mutations in vivo are not known. Previous studies have demonstrated hyperactivation of p85α regulatory subunit of class IA phosphatidylinositol-3-kinase (PI3K) in cell lines expressing the activation loop mutant of KIT (KITD816V [human] and KITD814V [murine]). Although p85α is hyperphosphorylated and constitutively bound to KITD814V in cell-line models; the physiologic significance of this biochemical phenomenon in KITD814V-induced transformation is not known. Materials and Methods: Here, we describe the generation of a new mouse model to study KITD814V-induced transformation in myeloid cells as opposed to previously described models that primarily result in the generation of disease resembling acute lymphocytic leukemia. Results: Our results show that transplantation of KITD814V expressing bone marrow cells from C57/BL6 strain of mice into syngeneic recipients results in a fatal MPD. Importantly, in this model, transplantation of KITD814V expressing p85α-deficient bone marrow cells rescues the MPD phenotype. Conclusions: Our results describe the generation of a new murine transplant model to study KITD814V-induced transformation and identify p85α as potential therapeutic target for the treatment of KITD814V-bearing diseases.

Original languageEnglish (US)
Pages (from-to)301-308
Number of pages8
JournalExperimental Hematology
Volume36
Issue number3
DOIs
StatePublished - Mar 1 2008

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ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

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