Requirement of the self-glucosylating initiator proteins Glg1p and Glg2p for glycogen accumulation in Saccharomyces cerevisiae

C. Cheng, J. Mu, I. Farkas, D. Huang, Mark Goebl, Peter Roach

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75 Citations (Scopus)

Abstract

Glycogen, a branched polymer of glucose, is a storage molecule whose accumulation is under rigorous nutritional control in many cells. We report the identification of two Saccharomyces cerevisiae genes, GLG1 and GLG2, whose products are implicated in the biogenesis of glycogen. These genes encode self-glucosylating proteins that in vitro can act as primers for the elongation reaction catalyzed by glycogen synthase. Over a region of 258 residues, the Gig proteins have 55% sequence identity to each other and ~33% identity to glycogenin, a mammalian protein postulated to have a role in the initiation of glycogen biosynthesis. Yeast cells defective in either GLG1 or GLG2 are similar to the wild type in their ability to accumulate glycogen. Disruption of both genes results in the inability of the cells to synthesize glycogen despite normal levels of glycogen synthase. These results suggest that a self-glucosylating protein is required for glycogen biosynthesis in a eukaryotic cell. The activation state of glycogen synthase in glg1 glg2 cells is suppressed, suggesting that the Gig proteins may additionally influence the phosphorylation state of glycogen synthase.

Original languageEnglish
Pages (from-to)6632-6640
Number of pages9
JournalMolecular and Cellular Biology
Volume15
Issue number12
StatePublished - 1995

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Glycogen
Saccharomyces cerevisiae
Glycogen Synthase
Proteins
Genes
Glucans
Eukaryotic Cells
Yeasts
Phosphorylation

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Requirement of the self-glucosylating initiator proteins Glg1p and Glg2p for glycogen accumulation in Saccharomyces cerevisiae. / Cheng, C.; Mu, J.; Farkas, I.; Huang, D.; Goebl, Mark; Roach, Peter.

In: Molecular and Cellular Biology, Vol. 15, No. 12, 1995, p. 6632-6640.

Research output: Contribution to journalArticle

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AU - Goebl, Mark

AU - Roach, Peter

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AB - Glycogen, a branched polymer of glucose, is a storage molecule whose accumulation is under rigorous nutritional control in many cells. We report the identification of two Saccharomyces cerevisiae genes, GLG1 and GLG2, whose products are implicated in the biogenesis of glycogen. These genes encode self-glucosylating proteins that in vitro can act as primers for the elongation reaction catalyzed by glycogen synthase. Over a region of 258 residues, the Gig proteins have 55% sequence identity to each other and ~33% identity to glycogenin, a mammalian protein postulated to have a role in the initiation of glycogen biosynthesis. Yeast cells defective in either GLG1 or GLG2 are similar to the wild type in their ability to accumulate glycogen. Disruption of both genes results in the inability of the cells to synthesize glycogen despite normal levels of glycogen synthase. These results suggest that a self-glucosylating protein is required for glycogen biosynthesis in a eukaryotic cell. The activation state of glycogen synthase in glg1 glg2 cells is suppressed, suggesting that the Gig proteins may additionally influence the phosphorylation state of glycogen synthase.

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