Resident c-kit + cells in the heart are not cardiac stem cells

Nishat Sultana, Lu Zhang, Jianyun Yan, Jiqiu Chen, Weibin Cai, Shegufta Razzaque, Dongtak Jeong, Wei Sheng, Lei Bu, Mingjiang Xu, Guo Ying Huang, Roger J. Hajjar, Bin Zhou, Anne Moon, Chen Leng Cai

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

Identifying a bona fide population of cardiac stem cells (CSCs) is a critical step for developing cell-based therapies for heart failure patients. Previously, cardiac c-kit + cells were reported to be CSCs with a potential to become myocardial, endothelial and smooth muscle cells in vitro and after cardiac injury. Here we provide further insights into the nature of cardiac c-kit + cells. By targeting the c-kit locus with multiple reporter genes in mice, we find that c-kit expression rarely co-localizes with the expression of the cardiac progenitor and myogenic marker Nkx2.5, or that of the myocardial marker, cardiac troponin T (cTnT). Instead, c-kit predominantly labels a cardiac endothelial cell population in developing and adult hearts. After acute cardiac injury, c-kit + cells retain their endothelial identity and do not become myogenic progenitors or cardiomyocytes. Thus, our work strongly suggests that c-kit + cells in the murine heart are endothelial cells and not CSCs.

Original languageEnglish (US)
Article number8701
JournalNature Communications
Volume6
DOIs
StatePublished - Oct 30 2015
Externally publishedYes

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kits
stem cells
Stem cells
Stem Cells
Endothelial cells
cells
Troponin T
Endothelial Cells
Muscle
muscles
Labels
Wounds and Injuries
markers
Cell- and Tissue-Based Therapy
Genes
Reporter Genes
Cells
Cardiac Myocytes
Population
Smooth Muscle Myocytes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Sultana, N., Zhang, L., Yan, J., Chen, J., Cai, W., Razzaque, S., ... Cai, C. L. (2015). Resident c-kit + cells in the heart are not cardiac stem cells. Nature Communications, 6, [8701]. https://doi.org/10.1038/ncomms9701

Resident c-kit + cells in the heart are not cardiac stem cells. / Sultana, Nishat; Zhang, Lu; Yan, Jianyun; Chen, Jiqiu; Cai, Weibin; Razzaque, Shegufta; Jeong, Dongtak; Sheng, Wei; Bu, Lei; Xu, Mingjiang; Huang, Guo Ying; Hajjar, Roger J.; Zhou, Bin; Moon, Anne; Cai, Chen Leng.

In: Nature Communications, Vol. 6, 8701, 30.10.2015.

Research output: Contribution to journalArticle

Sultana, N, Zhang, L, Yan, J, Chen, J, Cai, W, Razzaque, S, Jeong, D, Sheng, W, Bu, L, Xu, M, Huang, GY, Hajjar, RJ, Zhou, B, Moon, A & Cai, CL 2015, 'Resident c-kit + cells in the heart are not cardiac stem cells', Nature Communications, vol. 6, 8701. https://doi.org/10.1038/ncomms9701
Sultana N, Zhang L, Yan J, Chen J, Cai W, Razzaque S et al. Resident c-kit + cells in the heart are not cardiac stem cells. Nature Communications. 2015 Oct 30;6. 8701. https://doi.org/10.1038/ncomms9701
Sultana, Nishat ; Zhang, Lu ; Yan, Jianyun ; Chen, Jiqiu ; Cai, Weibin ; Razzaque, Shegufta ; Jeong, Dongtak ; Sheng, Wei ; Bu, Lei ; Xu, Mingjiang ; Huang, Guo Ying ; Hajjar, Roger J. ; Zhou, Bin ; Moon, Anne ; Cai, Chen Leng. / Resident c-kit + cells in the heart are not cardiac stem cells. In: Nature Communications. 2015 ; Vol. 6.
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