Resistance of cholestatic rats against epinephrine-induced arrhythmia: The role of nitric oxide and endogenous opioids

Amir R. Hajrasouliha, Sina Tavakoli, Pejman Jabehdar-Maralani, Hamed Shafaroodi, Amir Ali Borhani, Golbahar Houshmand, Hamed Sadeghipour, Mehdi Dehghani, Ahmad Reza Dehpour

Research output: Contribution to journalArticle

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Abstract

Short-term ligation of bile duct has been used as a model to study acute cholestasis and is associated with various cardiovascular abnormalities. We examined the role of nitric oxide (NO) and endogenous opioids on epinephrine-induced arrhythmia in 7-day bile duct-ligated (BDL) rats. Six groups of rats, each of which was subdivided into two subgroups (sham-operated and BDL), were examined. First group of animals were chronically treated with normal saline. In the second and third groups, single intraperitoneal administration of N(ω)-nitro-l-arginine methyl ester (l-NAME, 10 mg/kg) or naltrexone (20 mg/kg) was performed 30 min before evaluation of epinephrine-induced arrhythmia. Two groups received chronic administration of low dose (3 mg/kg/day) or high dose (10 mg/kg/day) l-NAME; and the last group was treated chronically with naltrexone (20 mg/kg/day). Chronic drug administration was performed subcutaneously for 6 consecutive days following BDL or sham operation. After induction of arrhythmia by intravenous injection of 10 μg/kg epinephrine, mean arterial pressure and electrocardiogram were recorded for 1 min. Heart rate and mean arterial pressure were significantly lower in BDL rats (P<0.01). Chronic injection of naltrexone increased heart rate and mean arterial pressure in BDL (P<0.05). Chronic low dose l-NAME administration had no effect on baseline hemodynamic parameters. High dose l-NAME injection corrected hypotension in BDL rats, but not bradycardia (P<0.05). Epinephrine induced less arrhythmia in BDL rats (P<0.05). Acute and chronic injection of naltrexone had no effect on the resistance of BDL rats against epinephrine-induced arrhythmia. Although acute l-NAME administration enhanced arrhythmias in sham-operated rats (P<0.001), it had no effect on BDL animals. Chronic injection of low dose or high dose l-NAME, without having any effect on sham-operated animals, increased arrhythmias in BDL rats (P<0.01). This study showed that BDL animals are resistant against epinephrine-induced arrhythmia and this resistance depends on long-term NO overproduction.

Original languageEnglish (US)
Pages (from-to)307-313
Number of pages7
JournalEuropean Journal of Pharmacology
Volume499
Issue number3
DOIs
StatePublished - Sep 24 2004
Externally publishedYes

Fingerprint

Bile Ducts
Opioid Analgesics
Epinephrine
Cardiac Arrhythmias
Nitric Oxide
Naltrexone
Arterial Pressure
Injections
Heart Rate
Cardiovascular Abnormalities
Cholestasis
Bradycardia
Intravenous Injections
Hypotension
Ligation
Electrocardiography
Hemodynamics

Keywords

  • (Rat)
  • Cholestasis
  • Endogenous opioids
  • Epinephrine-induced arrhythmia
  • NO (Nitric oxide)

ASJC Scopus subject areas

  • Pharmacology

Cite this

Resistance of cholestatic rats against epinephrine-induced arrhythmia : The role of nitric oxide and endogenous opioids. / Hajrasouliha, Amir R.; Tavakoli, Sina; Jabehdar-Maralani, Pejman; Shafaroodi, Hamed; Borhani, Amir Ali; Houshmand, Golbahar; Sadeghipour, Hamed; Dehghani, Mehdi; Dehpour, Ahmad Reza.

In: European Journal of Pharmacology, Vol. 499, No. 3, 24.09.2004, p. 307-313.

Research output: Contribution to journalArticle

Hajrasouliha, AR, Tavakoli, S, Jabehdar-Maralani, P, Shafaroodi, H, Borhani, AA, Houshmand, G, Sadeghipour, H, Dehghani, M & Dehpour, AR 2004, 'Resistance of cholestatic rats against epinephrine-induced arrhythmia: The role of nitric oxide and endogenous opioids', European Journal of Pharmacology, vol. 499, no. 3, pp. 307-313. https://doi.org/10.1016/j.ejphar.2004.07.099
Hajrasouliha, Amir R. ; Tavakoli, Sina ; Jabehdar-Maralani, Pejman ; Shafaroodi, Hamed ; Borhani, Amir Ali ; Houshmand, Golbahar ; Sadeghipour, Hamed ; Dehghani, Mehdi ; Dehpour, Ahmad Reza. / Resistance of cholestatic rats against epinephrine-induced arrhythmia : The role of nitric oxide and endogenous opioids. In: European Journal of Pharmacology. 2004 ; Vol. 499, No. 3. pp. 307-313.
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AU - Shafaroodi, Hamed

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N2 - Short-term ligation of bile duct has been used as a model to study acute cholestasis and is associated with various cardiovascular abnormalities. We examined the role of nitric oxide (NO) and endogenous opioids on epinephrine-induced arrhythmia in 7-day bile duct-ligated (BDL) rats. Six groups of rats, each of which was subdivided into two subgroups (sham-operated and BDL), were examined. First group of animals were chronically treated with normal saline. In the second and third groups, single intraperitoneal administration of N(ω)-nitro-l-arginine methyl ester (l-NAME, 10 mg/kg) or naltrexone (20 mg/kg) was performed 30 min before evaluation of epinephrine-induced arrhythmia. Two groups received chronic administration of low dose (3 mg/kg/day) or high dose (10 mg/kg/day) l-NAME; and the last group was treated chronically with naltrexone (20 mg/kg/day). Chronic drug administration was performed subcutaneously for 6 consecutive days following BDL or sham operation. After induction of arrhythmia by intravenous injection of 10 μg/kg epinephrine, mean arterial pressure and electrocardiogram were recorded for 1 min. Heart rate and mean arterial pressure were significantly lower in BDL rats (P<0.01). Chronic injection of naltrexone increased heart rate and mean arterial pressure in BDL (P<0.05). Chronic low dose l-NAME administration had no effect on baseline hemodynamic parameters. High dose l-NAME injection corrected hypotension in BDL rats, but not bradycardia (P<0.05). Epinephrine induced less arrhythmia in BDL rats (P<0.05). Acute and chronic injection of naltrexone had no effect on the resistance of BDL rats against epinephrine-induced arrhythmia. Although acute l-NAME administration enhanced arrhythmias in sham-operated rats (P<0.001), it had no effect on BDL animals. Chronic injection of low dose or high dose l-NAME, without having any effect on sham-operated animals, increased arrhythmias in BDL rats (P<0.01). This study showed that BDL animals are resistant against epinephrine-induced arrhythmia and this resistance depends on long-term NO overproduction.

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