Resistance vessel remodeling and reparative angiogenesis in the microcirculatory bed of long-term denervated skeletal muscles

E. I. Dedkov, Tatiana Kostrominova, A. B. Borisov, B. M. Carlson

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In denervated skeletal muscles, atrophy of muscle fibers and interstitial fibrosis are associated with alterations within the vascular bed. Our study has placed particular emphasis on changes occurring in resistance vessels and the microcirculatory bed of rat hindlimb skeletal muscles that had been denervated for 25 months. We found that the tunica media of the majority of long-term denervated resistance vessels undergoes deterioration. In small intramuscular arteries and arterioles, atrophic vascular smooth muscle cells (vSMCs) enclosed in a thick basal lamina are separated by expanded extracellular space. The remodeling and sclerotic changes in the arterial wall occasionally result in deformation of the lumen. It was also found that the microcirculatory bed undergoes significant alterations. In 25-month denervated extensor digitorum longus muscle, the capillary-to-fiber ratio is only 0.13 ± 0.01 and the mean number of capillaries per fascicle decreases almost ninefold compared to contralateral control muscle. Ultrastructural findings demonstrate that 24.67 ± 0.48% of capillaries examined in the chronically denervated fascicles show structural features typical for capillary regeneration. In addition, long cytoplasmic extensions of pericytes might develop a layer completely encircling the capillary endothelium. In pre-and postcapillary segments of the microcirculatory bed, some perivascular cells possess a phenotype that is intermediate between that of pericytes and atrophic vSMCs. RT-PCR and/or Western blot analyses showed that molecules participating in angiogenesis are detected in 25-month denervated skeletal muscle. We hypothesize that despite the fact that the microcirculatory bed of chronically denervated muscle undergoes significant reduction it still sustains the capacity for reparative capillary growth.

Original languageEnglish (US)
Pages (from-to)96-114
Number of pages19
JournalMicrovascular Research
Volume63
Issue number1
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Muscle
Skeletal Muscle
Muscles
Pericytes
Vascular Smooth Muscle
Smooth Muscle Myocytes
Tunica Media
Muscular Atrophy
Vascular Endothelium
Extracellular Space
Arterioles
Hindlimb
Basement Membrane
Blood Vessels
Regeneration
Fibrosis
Arteries
Western Blotting
Phenotype
Fibers

Keywords

  • Capillary regeneration
  • Flk-1/KDR
  • Flt-1
  • HIF-1α
  • Intramuscular resistance vessels
  • Microcirculatory bed
  • Prolonged muscle denervation
  • Sciatic nerve axotomy
  • VEGF

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine

Cite this

Resistance vessel remodeling and reparative angiogenesis in the microcirculatory bed of long-term denervated skeletal muscles. / Dedkov, E. I.; Kostrominova, Tatiana; Borisov, A. B.; Carlson, B. M.

In: Microvascular Research, Vol. 63, No. 1, 2002, p. 96-114.

Research output: Contribution to journalArticle

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