Resistin impairs endothelium-dependent dilation to bradykinin, but not acetylcholine, in the coronary circulation

Gregory M. Dick, Paige S. Katz, Martin Farias, Michael Morris, Jeremy James, Jarrod D. Knudson, Johnathan Tune

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Elevated plasma levels of fat-derived signaling molecules are associated with obesity, vascular endothelial dysfunction, and coronary heart disease; however, little is known about their direct coronary vascular effects. Accordingly, we examined mechanisms by which one adipokine, resistin, affects coronary vascular tone and endothelial function. Studies were conducted in anesthetized dogs and isolated coronary artery rings. Resistin did not change coronary blood flow, mean arterial pressure, or heart rate. Resistin had no effect on acetylcholine-induced relaxation of artery rings; however, resistin did impair bradykinin-induced relaxation. Selective impairment was also observed in vivo, as resistin attenuated vasodilation to bradykinin but not to acetylcholine. Resistin had no effect on dihydroethidium fluorescence, an indicator of superoxide (O2-) production, and the inhibitory effect of resistin on bradykinin-induced relaxation persisted in the presence of Tempol, a superoxide dismutase mimetic. To determine whether resistin impaired production of and/or responses to nitric oxide (NO) or prostaglandins (e.g., prostacyclin; PGI2), we performed experiments with Nω-nitro-L-arginine methyl ester (L-NAME) and indomethacin. The effect of resistin to attenuate bradykinin-induced vasodilation persisted in the presence of L-NAME or indomethacin, suggesting resistin may act at a cell signaling point upstream of NO or PGI2 production. Resistin-induced endothelial dysfunction is not generalized, and it is not consistent with effects mediated by O2- or interference with NO or PGI2 signaling. The site of the resistin-induced impairment is unknown but may be at the bradykinin receptor or a closely associated signal transduction machinery proximal to NO synthase or cyclooxygenase.

Original languageEnglish
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume291
Issue number6
DOIs
StatePublished - 2006

Fingerprint

Resistin
Coronary Circulation
Bradykinin
Acetylcholine
Endothelium
Dilatation
Epoprostenol
Blood Vessels
Nitric Oxide
NG-Nitroarginine Methyl Ester
Vasodilation
Indomethacin
Bradykinin Receptors
Adipokines
Prostaglandin-Endoperoxide Synthases
Nitric Oxide Synthase
Superoxides
Superoxide Dismutase
Prostaglandins
Coronary Disease

Keywords

  • Adipokine
  • Hormone
  • Obesity
  • Reactive oxygen species

ASJC Scopus subject areas

  • Physiology

Cite this

Resistin impairs endothelium-dependent dilation to bradykinin, but not acetylcholine, in the coronary circulation. / Dick, Gregory M.; Katz, Paige S.; Farias, Martin; Morris, Michael; James, Jeremy; Knudson, Jarrod D.; Tune, Johnathan.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 291, No. 6, 2006.

Research output: Contribution to journalArticle

Dick, Gregory M. ; Katz, Paige S. ; Farias, Martin ; Morris, Michael ; James, Jeremy ; Knudson, Jarrod D. ; Tune, Johnathan. / Resistin impairs endothelium-dependent dilation to bradykinin, but not acetylcholine, in the coronary circulation. In: American Journal of Physiology - Heart and Circulatory Physiology. 2006 ; Vol. 291, No. 6.
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