Resorption controls bone anabolism driven by parathyroid hormone (PTH) receptor signaling in osteocytes

Yumie Rhee, Eun Young Lee, Virginia Lezcano, Ana C. Ronda, Keith W. Condon, Matthew Allen, Lilian Plotkin, Teresita Bellido

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The contribution of remodeling-based bone formation coupled to osteoclast activity versus modeling-based bone formation that occurs independently of resorption, to the anabolic effect of PTH remains unclear. We addressed this question using transgenic mice with activated PTH receptor signaling in osteocytes that exhibit increased bone mass and remodeling, recognized skeletal effects of PTH elevation. Direct inhibition of bone formation was accomplished genetically by overexpressing the Wnt antagonist Sost/sclerostin; and resorption-dependent bone formation was inhibited pharmacologically with the bisphosphonate alendronate. We found that bone formation induced by osteocytic PTH receptor signaling on the periosteal surface depends on Wnt signaling but not on resorption. In contrast, bone formation on the endocortical surface results from a combination of Wntdriven increased osteoblast number and resorption-dependent osteoblast activity. Moreover, elevated osteoclasts and intracortical/calvarial porosity is exacerbated by overexpressing Sost and reversed by blocking resorption. Furthermore, increased cancellous bone is abolished by Wnt inhibition but further increased by blocking resorption. Thus, resorption induced by PTH receptor signaling in osteocytes is critical for full anabolism in cortical bone, but tempers bone gain in cancellous bone. Dissecting underlying mechanisms of PTH receptor signaling would allow targeting actions in different bone compartments, enhancing the therapeutic potential of the pathway.

Original languageEnglish
Pages (from-to)29809-29820
Number of pages12
JournalJournal of Biological Chemistry
Volume288
Issue number41
DOIs
StatePublished - Oct 11 2013

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Parathyroid Hormone Receptor Type 1
Osteocytes
Bone Resorption
Osteogenesis
Bone
Osteoclasts
Parathyroid Hormone
Osteoblasts
Anabolic Agents
Bone and Bones
Alendronate
Bone Remodeling
Porosity
Diphosphonates
Transgenic Mice

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Resorption controls bone anabolism driven by parathyroid hormone (PTH) receptor signaling in osteocytes. / Rhee, Yumie; Lee, Eun Young; Lezcano, Virginia; Ronda, Ana C.; Condon, Keith W.; Allen, Matthew; Plotkin, Lilian; Bellido, Teresita.

In: Journal of Biological Chemistry, Vol. 288, No. 41, 11.10.2013, p. 29809-29820.

Research output: Contribution to journalArticle

Rhee, Yumie ; Lee, Eun Young ; Lezcano, Virginia ; Ronda, Ana C. ; Condon, Keith W. ; Allen, Matthew ; Plotkin, Lilian ; Bellido, Teresita. / Resorption controls bone anabolism driven by parathyroid hormone (PTH) receptor signaling in osteocytes. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 41. pp. 29809-29820.
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