Respiratory syncytial virus causes increased bronchial epithelial permeability

Muna M. Kilani, Kamal A. Mohammed, Najmunnisa Nasreen, Joyce A. Hardwick, Mark Kaplan, Robert Tepper, Veena B. Antony

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: Respiratory syncytial virus (RSV)-induced diseases are mediated through active cytokines released during infection. We hypothesized that RSV infection causes bronchial epithelial monolayer permeability in vitro via induction of vascular endothelial growth factor (VEGF). Methods: Human bronchial epithelial cells were infected with RSV. In some cultures, VEGF antibody was included to block VEGF response; in other cultures, palivizumab was added to block RSV infection. Permeability was assessed in real-time using electric cell-substrate impedance sensing. VEGF release was assessed using enzyme-linked immunosorbent assay. Gap formation was assessed using live cell imaging. Results: RSV-infected cells demonstrated a decrease in the resistance of the monolayer indicating an increase in permeability; this increase was blocked with VEGF-specific antibody, and palivizumab. Intercellular gap formation developed in RSV-infected epithelial monolayers. Conclusion: RSV increases permeability of the bronchial airway epithelial monolayer via VEGF induction.

Original languageEnglish
Pages (from-to)186-191
Number of pages6
JournalChest
Volume126
Issue number1
DOIs
StatePublished - Jul 2004

Fingerprint

Respiratory Syncytial Viruses
Vascular Endothelial Growth Factor A
Permeability
Respiratory Syncytial Virus Infections
Antibodies
Virus Diseases
Electric Impedance
Epithelial Cells
Enzyme-Linked Immunosorbent Assay
Cytokines
Infection

Keywords

  • Edema
  • Electric cell-substrate impedance sensing
  • Respiratory syncytial virus
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Kilani, M. M., Mohammed, K. A., Nasreen, N., Hardwick, J. A., Kaplan, M., Tepper, R., & Antony, V. B. (2004). Respiratory syncytial virus causes increased bronchial epithelial permeability. Chest, 126(1), 186-191. https://doi.org/10.1378/chest.126.1.186

Respiratory syncytial virus causes increased bronchial epithelial permeability. / Kilani, Muna M.; Mohammed, Kamal A.; Nasreen, Najmunnisa; Hardwick, Joyce A.; Kaplan, Mark; Tepper, Robert; Antony, Veena B.

In: Chest, Vol. 126, No. 1, 07.2004, p. 186-191.

Research output: Contribution to journalArticle

Kilani, MM, Mohammed, KA, Nasreen, N, Hardwick, JA, Kaplan, M, Tepper, R & Antony, VB 2004, 'Respiratory syncytial virus causes increased bronchial epithelial permeability', Chest, vol. 126, no. 1, pp. 186-191. https://doi.org/10.1378/chest.126.1.186
Kilani, Muna M. ; Mohammed, Kamal A. ; Nasreen, Najmunnisa ; Hardwick, Joyce A. ; Kaplan, Mark ; Tepper, Robert ; Antony, Veena B. / Respiratory syncytial virus causes increased bronchial epithelial permeability. In: Chest. 2004 ; Vol. 126, No. 1. pp. 186-191.
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