Respiratory syncytial virus causes increased bronchial epithelial permeability

Muna M. Kilani, Kamal A. Mohammed, Najmunnisa Nasreen, Joyce A. Hardwick, Mark H. Kaplan, Robert S. Tepper, Veena B. Antony

Research output: Contribution to journalArticle

22 Scopus citations


Background: Respiratory syncytial virus (RSV)-induced diseases are mediated through active cytokines released during infection. We hypothesized that RSV infection causes bronchial epithelial monolayer permeability in vitro via induction of vascular endothelial growth factor (VEGF). Methods: Human bronchial epithelial cells were infected with RSV. In some cultures, VEGF antibody was included to block VEGF response; in other cultures, palivizumab was added to block RSV infection. Permeability was assessed in real-time using electric cell-substrate impedance sensing. VEGF release was assessed using enzyme-linked immunosorbent assay. Gap formation was assessed using live cell imaging. Results: RSV-infected cells demonstrated a decrease in the resistance of the monolayer indicating an increase in permeability; this increase was blocked with VEGF-specific antibody, and palivizumab. Intercellular gap formation developed in RSV-infected epithelial monolayers. Conclusion: RSV increases permeability of the bronchial airway epithelial monolayer via VEGF induction.

Original languageEnglish (US)
Pages (from-to)186-191
Number of pages6
Issue number1
StatePublished - Jul 2004


  • Edema
  • Electric cell-substrate impedance sensing
  • Respiratory syncytial virus
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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  • Cite this

    Kilani, M. M., Mohammed, K. A., Nasreen, N., Hardwick, J. A., Kaplan, M. H., Tepper, R. S., & Antony, V. B. (2004). Respiratory syncytial virus causes increased bronchial epithelial permeability. Chest, 126(1), 186-191.