Response of 7,12-dimethylbenz(a)anthracene-induced rat mammary tumor cells to tamoxifen, in vitro

R. J. Ballou, Ahmad Safa, M. T. Tseng

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Primary cell cultures from a density-defined cell subpopulation of the DMBA-induced rat mammary tumors were exposed to tamoxifen during their log phase of growth. Growth inhibition and the ultrastructure of surviving cells were examined along with the influence of the antiestrogen on the secreted proteins as determined by pulse labeling with [35S]methionine and fluorography. Cell growth was remarkably inhibited at clinically achievable concentrations. However, ultrastructural changes in the surviving cells were minimal, the most noteworthy being the accumulation of myelin bodies. Protein secretion was affected in the defined subpopulation of several tumors by the reduced production of a high-molecular-weight protein. These tumors may represent a population of estrogen-sensitive tumors within the DMBA-induced mammary tumor model.

Original languageEnglish (US)
Pages (from-to)256-263
Number of pages8
JournalProceedings of the Society for Experimental Biology and Medicine
Volume173
Issue number2
StatePublished - 1983
Externally publishedYes

Fingerprint

Tamoxifen
Rats
Tumors
9,10-Dimethyl-1,2-benzanthracene
Cells
Breast Neoplasms
Photofluorography
Growth
Neoplasms
Proteins
Estrogen Receptor Modulators
Primary Cell Culture
Vulnerable Populations
Myelin Sheath
Methionine
Estrogens
Cell Count
Molecular Weight
Cell growth
Cell culture

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Response of 7,12-dimethylbenz(a)anthracene-induced rat mammary tumor cells to tamoxifen, in vitro. / Ballou, R. J.; Safa, Ahmad; Tseng, M. T.

In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 173, No. 2, 1983, p. 256-263.

Research output: Contribution to journalArticle

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