Response of leptin to short-term and prolonged overfeeding in humans

Jerzy W. Kolaczynski, Joanna P. Ohannesian, Robert Considine, Cheryl C. Marco, José F. Caro

Research output: Contribution to journalArticle

342 Citations (Scopus)

Abstract

As one of the postulated roles of the ob gene product, leptin, is regulation of energy balance and preservation of normal body composition, we investigated the effect of acute and chronic calorie excess (weight gain) on serum leptin in humans. Two protocols were employed: 1) acute (12-h) massive (120 Cal/kg) voluntary overfeeding of eight healthy individuals; and 2) chronic overfeeding to attain 10% weight gain, with its subsequent maintenance for additional 2 weeks, involving six normal males. In the acute experiments (protocol 1), circulating leptin rose by 40% over baseline (P <0.01) during the final hours of overfeeding; this increase persisted until the next morning. At the point of achievement and the 2-week maintenance of 10% weight gain (protocol 2), a more than 3-fold rise in the basal leptin concentration was observed (P <0.01). A direct linear relationship was found between the magnitude of the leptin response to weight gain and the percent gain of body fat (r = 0.88; P <0.01). In summary, 1) in contrast to normal food intake (8), short term massive overfeeding is associated with a moderate elevation of circulating leptin levels that persists until next feeding cycle is initiated; and 2) a 10% weight gain causes different changes in the body composition, and the resulting rise in circulating leptin parallels the increase in the percentage of body fat. In conclusion, these studies document acute elevation of leptin in response to positive energy balance and suggest that developing resistance to leptin is associated with bigger fat deposition during weight gain in humans.

Original languageEnglish (US)
Pages (from-to)4162-4165
Number of pages4
JournalJournal of Clinical Endocrinology and Metabolism
Volume81
Issue number11
DOIs
StatePublished - 1996
Externally publishedYes

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Leptin
Weight Gain
Fats
Body Composition
Energy balance
Adipose Tissue
Maintenance
Chemical analysis
Eating

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Response of leptin to short-term and prolonged overfeeding in humans. / Kolaczynski, Jerzy W.; Ohannesian, Joanna P.; Considine, Robert; Marco, Cheryl C.; Caro, José F.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 81, No. 11, 1996, p. 4162-4165.

Research output: Contribution to journalArticle

Kolaczynski, Jerzy W. ; Ohannesian, Joanna P. ; Considine, Robert ; Marco, Cheryl C. ; Caro, José F. / Response of leptin to short-term and prolonged overfeeding in humans. In: Journal of Clinical Endocrinology and Metabolism. 1996 ; Vol. 81, No. 11. pp. 4162-4165.
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