Response of myogenic and fibrogenic cells to cytochalasin B and to colcemid. I. Light microscope observations

James Croop, H. Holtzer

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Cytochalasin B (CB) induces a biphasic retraction in some cell types. The rapid response that peaks in 30 min leads to the 'dendritic' condition. Replicating myogenic and fibrogenic cells, as well as postmitotic myoblasts and myotubes, participate in this reaction. This is followed by a slower phase that requires 40 hr for stabilization and leads to the fully 'arborized' state. Only replicating myogenic and fibrogenic cells participate in this reaction. Postmitotic myoblasts and myotubes do not arborize but round up and float off into the medium. Pretreatment with demecolcine does not block the rapid response to CB, but does block arborization. CB arborized cells exposed to demecolcine while in the presence of CB develop sufficient tension to pull themselves apart. If CB depolymerizes actin like filaments, and if such filaments constitute the only contractile system in the cell, then it is difficult to visualize how cells in CB develop such tension. Demecolcine induces twisting, birefringent bands in interphase and metaphase arrested myogenic and fibrogenic cells, and in postmitotic myotubes. Such bands are much more evident when CB arborized cells are removed from CB and allowed to relax in demecolcine. These birefringent bands assemble in the presence of cycloheximide, and may constitute 20% of the volume of the cell.

Original languageEnglish (US)
Pages (from-to)271-285
Number of pages15
JournalJournal of Cell Biology
Volume65
Issue number2
StatePublished - 1975
Externally publishedYes

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Demecolcine
Cytochalasin B
Light
Skeletal Muscle Fibers
Myoblasts
Interphase
Cycloheximide
Metaphase
Actin Cytoskeleton
Cell Size

ASJC Scopus subject areas

  • Cell Biology

Cite this

Response of myogenic and fibrogenic cells to cytochalasin B and to colcemid. I. Light microscope observations. / Croop, James; Holtzer, H.

In: Journal of Cell Biology, Vol. 65, No. 2, 1975, p. 271-285.

Research output: Contribution to journalArticle

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