Response of rainbow trout to constant-pressure and constant-volume hemorrhage

D. W. Duff, K. R. Olson

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19 Citations (Scopus)

Abstract

Plasma oncotic pressure (OC), protein concentration (P), osmolality (Osm), and hematocrit (Hct) were measured in conscious rainbow trout subjected to 120 min of constant-pressure (CPH) or constant-volume (CVH) hemorrhage. Blood volume, tissue red cell, and plasma space were determined in control and CPH trout with 51Cr-labeled red cells and 125I-albumin. At the onset of CPH , blood loss exceeded 1.5 ml · min-1 · kg-1 but quickly dropped to a constant rate of 0.1 ml · min-1 · kg-1 by 15 min. After 2 h, 73% of the initial blood volume was lost, and Hct, OC, and P were reduced by 55 ± 3, 39 ± 4, and 19 ± 9%, respectively; Osm did not change. Calculated blood volume was reduced by 39% (51Cr method) or by 56% (125I method). Red cell space was reduced in all tissues except brain. Plasma space decreased in stomach, increased in liver and kidney, and was unchanged in other tissues. CVH had similar effects on Hct, OC, and P, while Osm and plasma sodium increased. The time required for arterial pressure to recover to within 90% of control increased with successive bleeding from 39 to > 160 min. Twenty-four hours after CVH, Hct remained depressed, while all other parameters were returning toward control levels. Trout incorporate a variety of mechanisms to respond to hemorrhage, including mobilization of stored blood and reabsorption of fluid from interstitital and probably intracellular sources. High permeability of trout capillaries to protein may facilitate the restoration of protein and fluid and allow fish to survive extensive hypovolemia.

Original languageEnglish (US)
Pages (from-to)26/6
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume257
Issue number6
StatePublished - Dec 1 1989

Fingerprint

Oncorhynchus mykiss
Hematocrit
Trout
Blood Volume
Osmolar Concentration
Hemorrhage
Pressure
Hypovolemia
Proteins
Capillary Permeability
Plasma Cells
Albumins
Stomach
Arterial Pressure
Fishes
Sodium
Kidney
Liver
Brain

Keywords

  • capillary permeability
  • fluid restoration
  • hypovolemia
  • oncotic pressure
  • Starling forces

ASJC Scopus subject areas

  • Physiology

Cite this

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title = "Response of rainbow trout to constant-pressure and constant-volume hemorrhage",
abstract = "Plasma oncotic pressure (OC), protein concentration (P), osmolality (Osm), and hematocrit (Hct) were measured in conscious rainbow trout subjected to 120 min of constant-pressure (CPH) or constant-volume (CVH) hemorrhage. Blood volume, tissue red cell, and plasma space were determined in control and CPH trout with 51Cr-labeled red cells and 125I-albumin. At the onset of CPH , blood loss exceeded 1.5 ml · min-1 · kg-1 but quickly dropped to a constant rate of 0.1 ml · min-1 · kg-1 by 15 min. After 2 h, 73{\%} of the initial blood volume was lost, and Hct, OC, and P were reduced by 55 ± 3, 39 ± 4, and 19 ± 9{\%}, respectively; Osm did not change. Calculated blood volume was reduced by 39{\%} (51Cr method) or by 56{\%} (125I method). Red cell space was reduced in all tissues except brain. Plasma space decreased in stomach, increased in liver and kidney, and was unchanged in other tissues. CVH had similar effects on Hct, OC, and P, while Osm and plasma sodium increased. The time required for arterial pressure to recover to within 90{\%} of control increased with successive bleeding from 39 to > 160 min. Twenty-four hours after CVH, Hct remained depressed, while all other parameters were returning toward control levels. Trout incorporate a variety of mechanisms to respond to hemorrhage, including mobilization of stored blood and reabsorption of fluid from interstitital and probably intracellular sources. High permeability of trout capillaries to protein may facilitate the restoration of protein and fluid and allow fish to survive extensive hypovolemia.",
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AU - Olson, K. R.

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N2 - Plasma oncotic pressure (OC), protein concentration (P), osmolality (Osm), and hematocrit (Hct) were measured in conscious rainbow trout subjected to 120 min of constant-pressure (CPH) or constant-volume (CVH) hemorrhage. Blood volume, tissue red cell, and plasma space were determined in control and CPH trout with 51Cr-labeled red cells and 125I-albumin. At the onset of CPH , blood loss exceeded 1.5 ml · min-1 · kg-1 but quickly dropped to a constant rate of 0.1 ml · min-1 · kg-1 by 15 min. After 2 h, 73% of the initial blood volume was lost, and Hct, OC, and P were reduced by 55 ± 3, 39 ± 4, and 19 ± 9%, respectively; Osm did not change. Calculated blood volume was reduced by 39% (51Cr method) or by 56% (125I method). Red cell space was reduced in all tissues except brain. Plasma space decreased in stomach, increased in liver and kidney, and was unchanged in other tissues. CVH had similar effects on Hct, OC, and P, while Osm and plasma sodium increased. The time required for arterial pressure to recover to within 90% of control increased with successive bleeding from 39 to > 160 min. Twenty-four hours after CVH, Hct remained depressed, while all other parameters were returning toward control levels. Trout incorporate a variety of mechanisms to respond to hemorrhage, including mobilization of stored blood and reabsorption of fluid from interstitital and probably intracellular sources. High permeability of trout capillaries to protein may facilitate the restoration of protein and fluid and allow fish to survive extensive hypovolemia.

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