Response to methotrexate of cultured cells of the 7,12-dimethylbenz(α) anthracene-induced mammary tumor of rats

A kinetic and ultrastructural study

Ahmad Safa, M. T. Tseng

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The effect of methotrexate (MTX) on primary cultures of dimethylbenzenthracene (DMBA)-induced mammary tumors evaluated. Dose response curves and thymidine labelling indices from twelve tumors treated with 10-9 to 10-2M MTX varied considerably but showed three main patterns. At lower concentrations 10-9-10-8M, between 90-100% of cells survived 48 hours treatment. At 10-3M, only 50-60% of the cells remained. In 30% of the cultures, MTX was less toxic near saturation 10-2M than at 10-3M. This 'rebound' phenomenon was not observed in 60% of the cultures. The remaining 10% of tumors showed no response to MTX. Labelling index and cell survival correlated well in all experiments. As with cell kinetic data, a diversified ultrastructural response was also seen. Cultures exhibiting the 'rebound' phenomenon indistinguishable from controls. Compared with the sensitivity of different cell lines to MTX, DMBA-induced mammary tumor cells appear to be highly resistant to the drug.

Original languageEnglish (US)
Pages (from-to)522-523
Number of pages2
JournalIRCS Medical Science
Volume9
Issue number6
StatePublished - 1981
Externally publishedYes

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Methotrexate
Rats
Tumors
Cultured Cells
Cells
Breast Neoplasms
Kinetics
Labeling
Poisons
Thymidine
Neoplasms
Cell Survival
anthracene
Cell Line
Pharmaceutical Preparations
Experiments

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

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title = "Response to methotrexate of cultured cells of the 7,12-dimethylbenz(α) anthracene-induced mammary tumor of rats: A kinetic and ultrastructural study",
abstract = "The effect of methotrexate (MTX) on primary cultures of dimethylbenzenthracene (DMBA)-induced mammary tumors evaluated. Dose response curves and thymidine labelling indices from twelve tumors treated with 10-9 to 10-2M MTX varied considerably but showed three main patterns. At lower concentrations 10-9-10-8M, between 90-100{\%} of cells survived 48 hours treatment. At 10-3M, only 50-60{\%} of the cells remained. In 30{\%} of the cultures, MTX was less toxic near saturation 10-2M than at 10-3M. This 'rebound' phenomenon was not observed in 60{\%} of the cultures. The remaining 10{\%} of tumors showed no response to MTX. Labelling index and cell survival correlated well in all experiments. As with cell kinetic data, a diversified ultrastructural response was also seen. Cultures exhibiting the 'rebound' phenomenon indistinguishable from controls. Compared with the sensitivity of different cell lines to MTX, DMBA-induced mammary tumor cells appear to be highly resistant to the drug.",
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T2 - A kinetic and ultrastructural study

AU - Safa, Ahmad

AU - Tseng, M. T.

PY - 1981

Y1 - 1981

N2 - The effect of methotrexate (MTX) on primary cultures of dimethylbenzenthracene (DMBA)-induced mammary tumors evaluated. Dose response curves and thymidine labelling indices from twelve tumors treated with 10-9 to 10-2M MTX varied considerably but showed three main patterns. At lower concentrations 10-9-10-8M, between 90-100% of cells survived 48 hours treatment. At 10-3M, only 50-60% of the cells remained. In 30% of the cultures, MTX was less toxic near saturation 10-2M than at 10-3M. This 'rebound' phenomenon was not observed in 60% of the cultures. The remaining 10% of tumors showed no response to MTX. Labelling index and cell survival correlated well in all experiments. As with cell kinetic data, a diversified ultrastructural response was also seen. Cultures exhibiting the 'rebound' phenomenon indistinguishable from controls. Compared with the sensitivity of different cell lines to MTX, DMBA-induced mammary tumor cells appear to be highly resistant to the drug.

AB - The effect of methotrexate (MTX) on primary cultures of dimethylbenzenthracene (DMBA)-induced mammary tumors evaluated. Dose response curves and thymidine labelling indices from twelve tumors treated with 10-9 to 10-2M MTX varied considerably but showed three main patterns. At lower concentrations 10-9-10-8M, between 90-100% of cells survived 48 hours treatment. At 10-3M, only 50-60% of the cells remained. In 30% of the cultures, MTX was less toxic near saturation 10-2M than at 10-3M. This 'rebound' phenomenon was not observed in 60% of the cultures. The remaining 10% of tumors showed no response to MTX. Labelling index and cell survival correlated well in all experiments. As with cell kinetic data, a diversified ultrastructural response was also seen. Cultures exhibiting the 'rebound' phenomenon indistinguishable from controls. Compared with the sensitivity of different cell lines to MTX, DMBA-induced mammary tumor cells appear to be highly resistant to the drug.

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