Responses of CA1 pyramidal neurons in rat hippocampus to transient forebrain ischemia: An in vivo intracellular recording study

Zao C. Xu, William A. Pulsinelli

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The electrophysiological responses of CA1 pyramidal neurons to 5 min forebrain ischemia were studied with intracellular recording and staining techniques in vivo. The baseline membrane potential rapidly depolarized to approximately -20 mV about 3 min after the onset of ischemia and began to repolarize 1-3 min after recirculation. The amplitude of this ischemic depolarization (ID) was related directly to the severity of ischemia and its latency of onset was inversely related to brain temperature. Spontaneous synaptic activity ceased shortly after ischemia onset while the evoke synaptic potentials lasted until shortly before the onset of ID. Inhibitory postsynaptic potentials (IPSPs) disappeared earlier than excitatory postsynaptic potentials (EPSPs) and the membrane input resistance of CA1 neurons increased after the onset of ischemia.

Original languageEnglish (US)
Pages (from-to)187-191
Number of pages5
JournalNeuroscience Letters
Volume171
Issue number1-2
DOIs
StatePublished - Apr 25 1994
Externally publishedYes

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Pyramidal Cells
Prosencephalon
Hippocampus
Ischemia
Inhibitory Postsynaptic Potentials
Synaptic Potentials
Excitatory Postsynaptic Potentials
Membrane Potentials
Staining and Labeling
Neurons
Temperature
Membranes
Brain

Keywords

  • Cerebral ischemia
  • Hippocampus
  • In vivo preparation
  • Intracellular recording
  • Ischemic depolarization
  • Neurobiotin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Responses of CA1 pyramidal neurons in rat hippocampus to transient forebrain ischemia : An in vivo intracellular recording study. / Xu, Zao C.; Pulsinelli, William A.

In: Neuroscience Letters, Vol. 171, No. 1-2, 25.04.1994, p. 187-191.

Research output: Contribution to journalArticle

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