Responses of human neutrophils to nicotine and/or Porphyromonas gingivalis

Nouf K. Al-Shibani, Nawaf Y. Labban, Michael Kowolik, John D. Ruby, L. Windsor

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Tobacco smoking is considered a major modifiable risk factor for periodontal disease. Nicotine is the addictive ingredient in tobacco and has been shown to affect multiple cellular processes. Neutrophils are the first line of host defense and are critical cells in the maintenance of periodontal health through their role in the control of bacteria, but they can also contribute to the progression of periodontal disease by the production and release of reactive oxygen species (ROS). Virulence factors from periodontal pathogens, such as Porphyromonas gingivalis (Pg), stimulate the respiratory burst of neutrophils. The objective of this study is to explore the oxidative activity of neutrophils when stimulated with Pg, nicotine, or both. Methods: Neutrophils were separated from buffy coats by the double dextran gradient method. The generation of ROS by neutrophils was determined using luminol-dependent chemiluminescence assays. The reaction was followed for 90 minutes, and the neutrophil activation was recorded as the total integrated energy output. Results: The Pg and Pg plus nicotine groups had a significantly higher active and peak chemiluminescence than the nicotine group (all with P

Original languageEnglish (US)
Pages (from-to)1504-1508
Number of pages5
JournalJournal of Periodontology
Volume82
Issue number10
DOIs
StatePublished - Oct 2011

Fingerprint

Porphyromonas gingivalis
Nicotine
Neutrophils
Periodontal Diseases
Luminescence
Reactive Oxygen Species
Luminol
Neutrophil Activation
Respiratory Burst
Virulence Factors
Dextrans
Tobacco
Smoking
Bacteria
Health

Keywords

  • Neutrophils
  • Nicotine
  • Porphyromonas gingivalis
  • Reactive oxygen species

ASJC Scopus subject areas

  • Periodontics

Cite this

Responses of human neutrophils to nicotine and/or Porphyromonas gingivalis. / Al-Shibani, Nouf K.; Labban, Nawaf Y.; Kowolik, Michael; Ruby, John D.; Windsor, L.

In: Journal of Periodontology, Vol. 82, No. 10, 10.2011, p. 1504-1508.

Research output: Contribution to journalArticle

Al-Shibani, Nouf K. ; Labban, Nawaf Y. ; Kowolik, Michael ; Ruby, John D. ; Windsor, L. / Responses of human neutrophils to nicotine and/or Porphyromonas gingivalis. In: Journal of Periodontology. 2011 ; Vol. 82, No. 10. pp. 1504-1508.
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