Responses of leptin to short-term fasting and refeeding in humans: A link with ketogenesis but not ketones themselves

Jerzy W. Kolaczynski, Robert Considine, Joanna Ohannesian, Cheryl Marco, Irina Opentanova, Mark R. Nyce, Michael Myint, José F. Caro

Research output: Contribution to journalArticle

448 Citations (Scopus)

Abstract

We investigated the response of leptin to short-term fasting and refeeding in humans. A mild decline in subcutaneous adipocyte ob gene mRNA and a marked fall in serum leptin were observed after 36 and 60 h of fasting. The dynamics of the leptin decline and rise were further substantiated in a 6-day study consisting of a 36-h baseline period, followed by 36-h fast, and a subsequent refeeding with normal diet. Leptin began a steady decline from the baseline values after 12 h of fasting, reaching a nadir at 36 h. The subsequent restoration of normal food intake was associated with a prompt leptin rise and a return to baseline values 24 h later. When responses of leptin to fasting and refeeding were compared with that of glucose, insulin, fatty acids, and ketones, a reverse relationship between leptin and β-OH- butyrate was found. Consequently, we tested whether the reciprocal responses represented a causal relationship between leptin and β-OH-butyrate. Small amounts of infused glucose equal to the estimated contribution of gluconeogenesis, which was sufficient to prevent rise in ketogenesis, also prevented a fall in leptin. The infusion of β-OH-butyrate to produce hyperketonemia of the same magnitude as after a 36-h fast had no effect on leptin. The study indicates that one of the adaptive physiological responses to fasting is a fall in serum leptin. Although the mediator that brings about this effect remains unknown, it appears to be neither insulin nor ketones.

Original languageEnglish (US)
Pages (from-to)1511-1515
Number of pages5
JournalDiabetes
Volume45
Issue number11
StatePublished - 1996
Externally publishedYes

Fingerprint

Leptin
Ketones
Fasting
Butyrates
Insulin
Glucose
Gluconeogenesis
Serum
Adipocytes
Fatty Acids
Eating
Diet
Messenger RNA

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Kolaczynski, J. W., Considine, R., Ohannesian, J., Marco, C., Opentanova, I., Nyce, M. R., ... Caro, J. F. (1996). Responses of leptin to short-term fasting and refeeding in humans: A link with ketogenesis but not ketones themselves. Diabetes, 45(11), 1511-1515.

Responses of leptin to short-term fasting and refeeding in humans : A link with ketogenesis but not ketones themselves. / Kolaczynski, Jerzy W.; Considine, Robert; Ohannesian, Joanna; Marco, Cheryl; Opentanova, Irina; Nyce, Mark R.; Myint, Michael; Caro, José F.

In: Diabetes, Vol. 45, No. 11, 1996, p. 1511-1515.

Research output: Contribution to journalArticle

Kolaczynski, JW, Considine, R, Ohannesian, J, Marco, C, Opentanova, I, Nyce, MR, Myint, M & Caro, JF 1996, 'Responses of leptin to short-term fasting and refeeding in humans: A link with ketogenesis but not ketones themselves', Diabetes, vol. 45, no. 11, pp. 1511-1515.
Kolaczynski JW, Considine R, Ohannesian J, Marco C, Opentanova I, Nyce MR et al. Responses of leptin to short-term fasting and refeeding in humans: A link with ketogenesis but not ketones themselves. Diabetes. 1996;45(11):1511-1515.
Kolaczynski, Jerzy W. ; Considine, Robert ; Ohannesian, Joanna ; Marco, Cheryl ; Opentanova, Irina ; Nyce, Mark R. ; Myint, Michael ; Caro, José F. / Responses of leptin to short-term fasting and refeeding in humans : A link with ketogenesis but not ketones themselves. In: Diabetes. 1996 ; Vol. 45, No. 11. pp. 1511-1515.
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