Responsiveness of experimental prostate carcinoma bone tumors to neutron or photon radiation combined with cytokine therapy

Gilda G. Hillman, Richard L. Maughan, David J. Grignon, Mark Yudelev, Mingxin Che, Judith Abrams, Yu Wang, Andrey Layer, Jennifer L. Wright, Johanna Rubio, Jeffrey D. Forman

Research output: Contribution to journalArticle

3 Scopus citations


Purpose: To improve the outcome of radiotherapy for prostate carcinoma bone tumors, we investigated bone tumor irradiation with photons or neutrons followed by interleukin 2 (IL-2) therapy in a tumor model. Methods and Materials: Implantation of PC-3 cells in nude mouse femur cavity induced a bone tumor that progressed to the formation of a palpable tumor, at the hip joint, by Day 20. Established bone tumors were irradiated with photons or neutrons, and a day later, mice were treated with IL-2 therapy for 3 weekly cycles. Results: PC-3 bone tumors responded to radiation with photons or neutrons in a dose-dependent manner. Combination of photon or neutron radiation with IL-2 therapy increased tumor growth delay, compared to that with photons or neutrons alone. Radiation alone or combined with IL-2 significantly increased mouse survival compared to that with IL-2 or no treatment. After combined therapy, a complete inhibition of bone tumor growth was observed in 45% to 50% of the mice. Histologically, the combined therapy resulted in greater tumor destruction associated with fibrosis, new bone formation, and inflammatory infiltrates than that observed with each modality alone. Conclusions: The efficacy of tumor irradiation with neutrons or photons was enhanced by IL-2 therapy for the treatment of prostate carcinoma bone tumors.

Original languageEnglish (US)
Pages (from-to)1426-1437
Number of pages12
JournalInternational Journal of Radiation Oncology Biology Physics
Issue number5
StatePublished - Aug 1 2003



  • Interleukin-2
  • Mouse bone tumors
  • Radiation

ASJC Scopus subject areas

  • Radiation
  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

Cite this

Hillman, G. G., Maughan, R. L., Grignon, D. J., Yudelev, M., Che, M., Abrams, J., Wang, Y., Layer, A., Wright, J. L., Rubio, J., & Forman, J. D. (2003). Responsiveness of experimental prostate carcinoma bone tumors to neutron or photon radiation combined with cytokine therapy. International Journal of Radiation Oncology Biology Physics, 56(5), 1426-1437.