Restoration of responsiveness of chronic myeloid leukemia granulocyte-macrophage colony-forming cells to growth regulation in vitro following preincubation with prostaglandin E

L. M. Pelus, E. Gold, S. Saletan, M. Coleman

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Abstract

The ability to modulate granulocyte-macrophage colony-forming unit (CFU-GM) Ia-antigen expression and response to growth inhibition in vitro was investigated in normals and patients with chronic myeloid leukemia (CML). The hyporesponsiveness of CML CFU-GM to inhibition by prostaglandin E and acidic isoferritins in vitro and their associated diminished capacity for Ia-antigen expression could be reversed following suspension culture of bone marrow cells in the presence of prostaglandin E prior to soft agar culture. Suspension preculture with prostaglandin E for 24 hr resulted in the detection of a population of CFU-GM that were equivalent to normal CFU-GM in both response to inhibition by prostaglandin E and acid isoferritins and in their pattern of Ia-antigen expression. Cytogenetic analysis of the progeny of CFU-GM proliferating in cultures established from marrow cells, cultured directly upon isolation or following suspension culture in the absence or presence of prostaglandin E for 24 hr, indicated that the responding cell populations belonged to the Ph1-positive leukemic clone. Antigen detection on these CFU-GM resulted both from Ia-antigen reexpression and the induction of noncycling cells into S-phase with coincident expression of Ia-antigens. These studies provide further evidence for a direct regulatory association between Ia-antigen and control of granulocyte-macrophage progenitor cell proliferation, offer a possible explanation for the disordered regulatory responses observed in patients with CML, and indicate that abnormal growth phenotypes can be modulated, at least in vitro.

Original languageEnglish (US)
Pages (from-to)158-165
Number of pages8
JournalBlood
Volume62
Issue number1
StatePublished - Jan 1 1983

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Granulocyte-Macrophage Progenitor Cells
Macrophages
Histocompatibility Antigens Class II
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Prostaglandins E
Granulocytes
Restoration
Growth
Cells
Cell culture
Suspensions
Cell proliferation
Ferritins
Agar
In Vitro Techniques
Cytogenetic Analysis
Bone
S Phase
Bone Marrow Cells
Population

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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Restoration of responsiveness of chronic myeloid leukemia granulocyte-macrophage colony-forming cells to growth regulation in vitro following preincubation with prostaglandin E. / Pelus, L. M.; Gold, E.; Saletan, S.; Coleman, M.

In: Blood, Vol. 62, No. 1, 01.01.1983, p. 158-165.

Research output: Contribution to journalArticle

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