Restriction of foamy viruses by primate trim5α

Melvyn W. Yap, Dirk Lindemann, Nicole Stanke, Juliane Reh, Dana Westphal, Helmut Hanenberg, Sadayuki Ohkura, Jonathan P. Stoye

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Foamy viruses (FVs) are unconventional retroviruses with a replication strategy that is significantly different from orthoretroviruses and bears some homology to that of hepadnaviruses. Although some cellular proteins, such as APOBEC3, have been reported to block FVs, no restriction by Trim5α has been described to date. The sensitivity of three FV isolates of human-chimpanzee or prototypic (PFV), macaque (SFVmac), and feline (FFV) origin to a variety of primate Trim5αs was therefore tested. PFV and SFVmac were restricted by Trim5αs from most New World monkeys, but not from other primates, whereas FFV-based vectors were restricted by Trim5αs from the great apes gorilla and orangutan. Trim5αs from Old World monkeys did not restrict any FV isolate tested. Capuchin Trim5α was unique, as it restricted SFVmac and FFV but not PFV. Trim5α specificity for FVs was determined by the B30.2 domain, interestingly involving, in some instances, the same residues of the variable regions previously implicated as major determinants for human immunodeficiency virus type 1 restriction. FVs with chimeric Gags were made to map the viral determinants of sensitivity to restriction. The N-terminal half of the Gag molecule was found to contain the regions that control susceptibility. This region most likely corresponds to the capsid of conventional retroviruses. Due to their unique replication strategy, FVs should provide a valuable new system to examine the mechanism of retroviral restriction by Trim5α.

Original languageEnglish
Pages (from-to)5429-5439
Number of pages11
JournalJournal of Virology
Volume82
Issue number11
DOIs
StatePublished - Jun 2008

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Spumavirus
Primates
viruses
Retroviridae
Simian foamy virus
Hepadnaviridae
Pongo
Platyrrhini
Cercopithecidae
Gorilla gorilla
Capsid
Felidae
Hominidae
Macaca
Cebidae
Pongo pygmaeus
capsid
Gorilla
Pongidae
Human immunodeficiency virus 1

ASJC Scopus subject areas

  • Immunology

Cite this

Yap, M. W., Lindemann, D., Stanke, N., Reh, J., Westphal, D., Hanenberg, H., ... Stoye, J. P. (2008). Restriction of foamy viruses by primate trim5α. Journal of Virology, 82(11), 5429-5439. https://doi.org/10.1128/JVI.02462-07

Restriction of foamy viruses by primate trim5α. / Yap, Melvyn W.; Lindemann, Dirk; Stanke, Nicole; Reh, Juliane; Westphal, Dana; Hanenberg, Helmut; Ohkura, Sadayuki; Stoye, Jonathan P.

In: Journal of Virology, Vol. 82, No. 11, 06.2008, p. 5429-5439.

Research output: Contribution to journalArticle

Yap, MW, Lindemann, D, Stanke, N, Reh, J, Westphal, D, Hanenberg, H, Ohkura, S & Stoye, JP 2008, 'Restriction of foamy viruses by primate trim5α', Journal of Virology, vol. 82, no. 11, pp. 5429-5439. https://doi.org/10.1128/JVI.02462-07
Yap MW, Lindemann D, Stanke N, Reh J, Westphal D, Hanenberg H et al. Restriction of foamy viruses by primate trim5α. Journal of Virology. 2008 Jun;82(11):5429-5439. https://doi.org/10.1128/JVI.02462-07
Yap, Melvyn W. ; Lindemann, Dirk ; Stanke, Nicole ; Reh, Juliane ; Westphal, Dana ; Hanenberg, Helmut ; Ohkura, Sadayuki ; Stoye, Jonathan P. / Restriction of foamy viruses by primate trim5α. In: Journal of Virology. 2008 ; Vol. 82, No. 11. pp. 5429-5439.
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