Restructuring of the gut microbiome by intermittent fasting prevents retinopathy and prolongs survival in db/db mice

Eleni Beli, Yuanqing Yan, Leni Moldovan, Cristiano P. Vieira, Ruli Gao, Yaqian Duan, Ram Prasad, Ashay Bhatwadekar, Fletcher White, Steven D. Townsend, Luisa Chan, Caitlin N. Ryan, Daniel Morton, Emil G. Moldovan, Fang I. Chu, Gavin Y. Oudit, Hartmut Derendorf, Luciano Adorini, Xiaoxin X. Wang, Carmella Evans-MolinaRaghu Mirmira, Michael E. Boulton, Mervin Yoder, Qiuhong Li, Moshe Levi, Julia V. Busik, Maria B. Grant

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Intermittent fasting (IF) protects against the development of metabolic diseases and cancer, but whether it can prevent diabetic microvascular complications is not known. In db/db mice, we examined the impact of long-term IF on diabetic retinopathy (DR). Despite no change in glycated hemoglobin, db/db mice on the IF regimen displayed significantly longer survival and a reduction in DR end points, including acellular capillaries and leukocyte infiltration. We hypothesized that IF-mediated changes in the gut microbiota would produce beneficial metabolites and prevent the development of DR. Microbiome analysis revealed increased levels of Firmicutes and decreased Bacteroidetes and Verrucomicrobia. Compared with db/db mice on ad libitum feeding, changes in the microbiome of the db/db mice on IF were associated with increases in gut mucin, goblet cell number, villi length, and reductions in plasma peptidoglycan. Consistent with the known modulatory effects of Firmicutes on bile acid.

Original languageEnglish (US)
Pages (from-to)1867-1879
Number of pages13
JournalDiabetes
Volume67
Issue number9
DOIs
StatePublished - Sep 1 2018

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Fasting
Diabetic Retinopathy
Microbiota
Verrucomicrobia
Bacteroidetes
Goblet Cells
Peptidoglycan
Metabolic Diseases
Glycosylated Hemoglobin A
Mucins
Diabetes Complications
Bile Acids and Salts
Leukocytes
Cell Count
Gastrointestinal Microbiome
Neoplasms
Firmicutes

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Beli, E., Yan, Y., Moldovan, L., Vieira, C. P., Gao, R., Duan, Y., ... Grant, M. B. (2018). Restructuring of the gut microbiome by intermittent fasting prevents retinopathy and prolongs survival in db/db mice. Diabetes, 67(9), 1867-1879. https://doi.org/10.2337/db18-0158

Restructuring of the gut microbiome by intermittent fasting prevents retinopathy and prolongs survival in db/db mice. / Beli, Eleni; Yan, Yuanqing; Moldovan, Leni; Vieira, Cristiano P.; Gao, Ruli; Duan, Yaqian; Prasad, Ram; Bhatwadekar, Ashay; White, Fletcher; Townsend, Steven D.; Chan, Luisa; Ryan, Caitlin N.; Morton, Daniel; Moldovan, Emil G.; Chu, Fang I.; Oudit, Gavin Y.; Derendorf, Hartmut; Adorini, Luciano; Wang, Xiaoxin X.; Evans-Molina, Carmella; Mirmira, Raghu; Boulton, Michael E.; Yoder, Mervin; Li, Qiuhong; Levi, Moshe; Busik, Julia V.; Grant, Maria B.

In: Diabetes, Vol. 67, No. 9, 01.09.2018, p. 1867-1879.

Research output: Contribution to journalArticle

Beli, E, Yan, Y, Moldovan, L, Vieira, CP, Gao, R, Duan, Y, Prasad, R, Bhatwadekar, A, White, F, Townsend, SD, Chan, L, Ryan, CN, Morton, D, Moldovan, EG, Chu, FI, Oudit, GY, Derendorf, H, Adorini, L, Wang, XX, Evans-Molina, C, Mirmira, R, Boulton, ME, Yoder, M, Li, Q, Levi, M, Busik, JV & Grant, MB 2018, 'Restructuring of the gut microbiome by intermittent fasting prevents retinopathy and prolongs survival in db/db mice', Diabetes, vol. 67, no. 9, pp. 1867-1879. https://doi.org/10.2337/db18-0158
Beli, Eleni ; Yan, Yuanqing ; Moldovan, Leni ; Vieira, Cristiano P. ; Gao, Ruli ; Duan, Yaqian ; Prasad, Ram ; Bhatwadekar, Ashay ; White, Fletcher ; Townsend, Steven D. ; Chan, Luisa ; Ryan, Caitlin N. ; Morton, Daniel ; Moldovan, Emil G. ; Chu, Fang I. ; Oudit, Gavin Y. ; Derendorf, Hartmut ; Adorini, Luciano ; Wang, Xiaoxin X. ; Evans-Molina, Carmella ; Mirmira, Raghu ; Boulton, Michael E. ; Yoder, Mervin ; Li, Qiuhong ; Levi, Moshe ; Busik, Julia V. ; Grant, Maria B. / Restructuring of the gut microbiome by intermittent fasting prevents retinopathy and prolongs survival in db/db mice. In: Diabetes. 2018 ; Vol. 67, No. 9. pp. 1867-1879.
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