Retinal and preretinal localisation of epidermal growth factor, transforming growth factor alpha, and their receptor in proliferative diabetic retinopathy

Bhartiben Patel, Paul Hiscott, David Charteris, Joanne Mather, David McLeod, Mike Boulton

Research output: Contribution to journalArticle

44 Scopus citations


A number of growth factors have been implicated in the development and perpetuation of preretinal fibrovascular membranes in patients with proliferative diabetic retinopathy (PDR). The aim of this study was to determine the potential role of epidermal growth factor (EGF), transforming growth factor alpha (TGF-α), and their receptor (EGF-R) in PDR development. Immunostaining for EGF, TGF-α, and EGF-R was compared between normal retina, PDR retina, and PDR preretinal membranes. Weak staining for EGF and EGF-R was observed throughout the neural retina from non-diabetic eyes while weak to moderate staining for TGF-α was observed in the ganglion cell layer and the inner and outer nuclear layers. In contrast, intense staining for EGF and TGF-α and moderate staining for EGF-R were observed throughout the PDR retina. Immunoreactivity for EGF, TGF-α, and EGF-R was seen in the majority of the 11 excised membranes studied and, though variable, was generally greater than that observed in normal retinas. These results suggest an autocrine/paracrine role for EGF, TGF-α, and EGF-R in PDR.

Original languageEnglish (US)
Pages (from-to)714-718
Number of pages5
JournalBritish Journal of Ophthalmology
Issue number9
StatePublished - Jan 1 1994


ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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