Retinoic acid-induced expression of CD38 antigen in myeloid cells is mediated through retinoic acid receptor-α

Johannes Drach, Teresa McQueen, Heike Engel, Michael Andreeff, Kent Robertson, Steven J. Collins, Fabio Malavasi, Kapil Mehta

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

CD38 is a leukocyte differentiation antigen that has been thought to be a phenotypic marker of different subpopulations of T-and B-lymphocytes. In myeloid cells, CD38 is expressed during early stages of differentiation. Virtually no information is available on regulation and functions of CD38. Recently we reported that all-trans-retinoic acid (ATRA) is a potent and highly specific inducer of CD38 expression in human promyelocytic leukemia cells. Here we report that ATRA-induced expression of CD38 antigen in myeloid cells is mediated through retinoic acid-α receptor (RARα). ATRA failed to induce CD38 expression in a mutant subclone of the HL-60 myeloid leukemia cell line (designated HL-60R) that is relatively resistant to ATRA-induced granulocytic differentiation. Retroviral vector-mediated transduction of RA receptor (RARα) into this HL-60R subclone completely restored the sensitivity of these cells to ATRA in terms of their ability to express CD38. In contrast, CD38 expression was not inducible by ATRA in HL-60R cells, transfected with a functional RARβ, RARγ, or RXRα receptor. Induction of CD38 in acute promyelocytic and acute myeloblastic leukemia cells was independent of ATRA-induced cytodifferentiation. Following culture with ATRA, increased CD38 protein levels were also observed in normal CD34+ bone marrow cells, but not on normal circulating granulocytes. From these results, we conclude that CD38 is ATRA inducible in myeloid leukemia cells and normal CD34+ bone marrow cells. This effect is independent of differentiation and is mediated by RARα in HL-60 cells, suggesting a similar role for RARα in CD38 expression in other hematopoietic cells.

Original languageEnglish (US)
Pages (from-to)1746-1752
Number of pages7
JournalCancer Research
Volume54
Issue number7
StatePublished - Apr 1 1994
Externally publishedYes

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CD38 Antigens
Retinoic Acid Receptors
Myeloid Cells
Tretinoin
Myeloid Leukemia
HL-60 Cells
Bone Marrow Cells
Differentiation Antigens
HLA Antigens
Granulocytes
Acute Myeloid Leukemia
Leukemia
B-Lymphocytes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Drach, J., McQueen, T., Engel, H., Andreeff, M., Robertson, K., Collins, S. J., ... Mehta, K. (1994). Retinoic acid-induced expression of CD38 antigen in myeloid cells is mediated through retinoic acid receptor-α. Cancer Research, 54(7), 1746-1752.

Retinoic acid-induced expression of CD38 antigen in myeloid cells is mediated through retinoic acid receptor-α. / Drach, Johannes; McQueen, Teresa; Engel, Heike; Andreeff, Michael; Robertson, Kent; Collins, Steven J.; Malavasi, Fabio; Mehta, Kapil.

In: Cancer Research, Vol. 54, No. 7, 01.04.1994, p. 1746-1752.

Research output: Contribution to journalArticle

Drach, J, McQueen, T, Engel, H, Andreeff, M, Robertson, K, Collins, SJ, Malavasi, F & Mehta, K 1994, 'Retinoic acid-induced expression of CD38 antigen in myeloid cells is mediated through retinoic acid receptor-α', Cancer Research, vol. 54, no. 7, pp. 1746-1752.
Drach, Johannes ; McQueen, Teresa ; Engel, Heike ; Andreeff, Michael ; Robertson, Kent ; Collins, Steven J. ; Malavasi, Fabio ; Mehta, Kapil. / Retinoic acid-induced expression of CD38 antigen in myeloid cells is mediated through retinoic acid receptor-α. In: Cancer Research. 1994 ; Vol. 54, No. 7. pp. 1746-1752.
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