Retroviral-Mediated Gene Transfer

Jeanne R. McLachlin, Kenneth Cornetta, Martin A. Eglitis, W. French Anderson

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

This chapter discusses on the vector design, development of different packaging cell lines, and employment of single and multiple gene vectors in different model systems. Several retroviruses have been used to generate recombinant gene transfer vectors. These include Rous sarcoma virus (RSV), reticuloendotheliosis virus (Rev), murine mammary tumor virus (Mu- MTV), murine myeloproliferative sarcoma virus (MuMPSV), and Harvey murine sarcoma virus (HaMuSV). Gene transfer by retroviral vectors has become a widely used approach to study expression of foreign gene sequences in mammalian cells. Although other methods of gene transfer exist, investigators have taken advantage of both the structure and the life cycle of retroviruses to achieve efficient transduction of cells. This chapter addresses some of the issues concerning the use of recombinant retroviruses for human gene therapy. Many questions are still unresolved concerning the efficiency of transduction, attaining appropriate levels of expression, the possibility of specific cell targeting, and long-term safety questions that arise when this form of therapy is considered for use in the humans.

Original languageEnglish (US)
Pages (from-to)91-135
Number of pages45
JournalProgress in nucleic acid research and molecular biology
Volume38
Issue numberC
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology

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