Retroviral vector integration in post-transplant hematopoiesis in mice conditioned with either submyeloablative or ablative irradiation

M. A. Sadat, S. Dirscherl, L. Sastry, J. Dantzer, N. Pech, S. Griffin, T. Hawkins, Y. Zhao, C. N. Barese, S. Cross, A. Orazi, C. An, W. S. Goebel, M. C. Yoder, X. Li, M. Grez, K. Cornetta, S. D. Mooney, M. C. Dinauer

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

X-linked chronic granulomatous disease (X-CGD) is an inherited immunodeficiency with absent phagocyte NADPH-oxidase activity caused by defects in the gene-encoding gp91 phox. Here, we evaluated strategies for less intensive conditioning for gene therapy of genetic blood disorders without selective advantage for gene correction, such as might be used in a human X-CGD protocol. We compared submyeloablative with ablative irradiation as conditioning in murine X-CGD, examining engraftment, oxidase activity and vector integration in mice transplanted with marrow transduced with a γ-retroviral vector for gp91 phox expression. The frequency of oxidase-positive neutrophils in the donor population was unexpectedly higher in many 300 cGy-conditioned mice compared with lethally irradiated recipients, as was the fraction of vector-marked donor secondary CFU-S12. Vector integration sites in marrow, spleen and secondary CFU-S12 DNA from primary recipients were enriched for cancer-associated genes, including Evi1, and integrations in or near cancer-associated genes were more frequent in marrow and secondary CFU-S12 from 300 cGy-conditioned mice compared with fully ablated mice. These findings support the concept that vector integration can confer a selection bias, and suggest that the intensity of the conditioning regimen may further influence the effects of vector integration on clonal selection in post-transplant engraftment and hematopoiesis.

Original languageEnglish (US)
Pages (from-to)1452-1464
Number of pages13
JournalGene Therapy
Volume16
Issue number12
DOIs
StatePublished - Dec 3 2009

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Keywords

  • Chronic granulomatous disease
  • Insertional mutagenesis
  • NADPH oxidase
  • Retrovirus

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

Cite this

Sadat, M. A., Dirscherl, S., Sastry, L., Dantzer, J., Pech, N., Griffin, S., Hawkins, T., Zhao, Y., Barese, C. N., Cross, S., Orazi, A., An, C., Goebel, W. S., Yoder, M. C., Li, X., Grez, M., Cornetta, K., Mooney, S. D., & Dinauer, M. C. (2009). Retroviral vector integration in post-transplant hematopoiesis in mice conditioned with either submyeloablative or ablative irradiation. Gene Therapy, 16(12), 1452-1464. https://doi.org/10.1038/gt.2009.96