Reverse phase HPLC determination of 5,6-dihydro-5-azacytidine in biological fluids

P. N. Huguenin, H. N. Jayaram, J. A. Kelley

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A sensitive and specific reverse phase HPLC method which allows measurement of the new antitumor agent 5,6-dihydro-5-azacytidine (DHAC) in biological fluids at concentrations as low as 50 ng/ml (2 multiplied by 10** minus **7 M) has been developed. After addition of 5 prime -chloro-5 prime -deoxy-5,6-dihydro-5-azacytidine as an internal standard, sequential ultrafiltration, boronate gel affinity chromatography and cation exchange chromatography are employed to isolate DHAC from plasma or urine.

Original languageEnglish (US)
Pages (from-to)1433-1453
Number of pages21
JournalJournal of Liquid Chromatography
Volume7
Issue number7
StatePublished - 1984
Externally publishedYes

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5,6-dihydro-5-azacytidine
High Pressure Liquid Chromatography
Fluids
Affinity chromatography
Ultrafiltration
Chromatography
Affinity Chromatography
Antineoplastic Agents
Gel Chromatography
Cations
Gels
Urine
Plasmas

ASJC Scopus subject areas

  • Molecular Medicine

Cite this

Huguenin, P. N., Jayaram, H. N., & Kelley, J. A. (1984). Reverse phase HPLC determination of 5,6-dihydro-5-azacytidine in biological fluids. Journal of Liquid Chromatography, 7(7), 1433-1453.

Reverse phase HPLC determination of 5,6-dihydro-5-azacytidine in biological fluids. / Huguenin, P. N.; Jayaram, H. N.; Kelley, J. A.

In: Journal of Liquid Chromatography, Vol. 7, No. 7, 1984, p. 1433-1453.

Research output: Contribution to journalArticle

Huguenin, PN, Jayaram, HN & Kelley, JA 1984, 'Reverse phase HPLC determination of 5,6-dihydro-5-azacytidine in biological fluids', Journal of Liquid Chromatography, vol. 7, no. 7, pp. 1433-1453.
Huguenin, P. N. ; Jayaram, H. N. ; Kelley, J. A. / Reverse phase HPLC determination of 5,6-dihydro-5-azacytidine in biological fluids. In: Journal of Liquid Chromatography. 1984 ; Vol. 7, No. 7. pp. 1433-1453.
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