Reversible changes in the nuclear lamina induced by hyperthermia

Elizabeth A. Falloon, Joseph R. Dynlacht

Research output: Contribution to journalArticle

5 Scopus citations


The nuclear matrix (NM) has been identified as a potential target for heat-induced cell killing. Previous studies have shown that heat-shock may significantly modulate lamin B content. Since changes in NM structure have often been accompanied by changes in protein composition, we investigated whether hyperthermia induced changes in nuclear lamina (NL) structure in non-tolerant and thermotolerant cells, and the implications of these changes on cell survival. Using indirect immunofluorescence techniques and confocal microscopy, we found that heating cells at 42 or 45.5°C caused invaginations and other distortions of the peripheral NL. While hyperthermia did not alter the number or structure of internal lamin B foci, heat-induced alterations to the peripheral NL were dose-dependent. Interestingly, NL structure recovered with time after heating in cells that were destined to live or die. Thermotolerant cells heated at 45.5°C showed similar initial changes in the NL compared to non-tolerant cells, but recovery occurred much faster. Taken together, these results suggest that the amount of initial damage to the peripheral NL is not correlated with heat-induced cell killing. However, the possibility that an increased rate of recovery might confer a survival advantage cannot be discounted.

Original languageEnglish (US)
Pages (from-to)451-460
Number of pages10
JournalJournal of Cellular Biochemistry
Issue number3
StatePublished - 2002


  • Confocal microscopy
  • Heat shock proteins
  • Hyperthermia
  • Lamin B
  • Nuclear matrix

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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