Revised Subunit Structure of Yeast Transcription Factor IIH (TFIIH) and Reconciliation with Human TFIIH

Yuichiro Takagi, Hirofumi Komori, Wei Hau Chang, Andy Hudmon, Hediye Erdjument-Bromage, Paul Tempst, Roger D. Kornberg

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Tfb4 is identified as a subunit of the core complex of yeast RNA polymerase II general transcription factor IIH (TFIIH) by affinity purification, by peptide sequence analysis, and by expression of the entire complex in insect cells. Tfb3, previously identified as a component of the core complex, is shown instead to form a complex with cdk and cyclin subunits of TFIIH. This reassignment of subunits resolves a longstanding discrepancy between yeast and human TFIIH complexes.

Original languageEnglish (US)
Pages (from-to)43897-43900
Number of pages4
JournalJournal of Biological Chemistry
Volume278
Issue number45
DOIs
StatePublished - Nov 7 2003
Externally publishedYes

Fingerprint

Transcription Factor TFIIH
Yeast
Yeasts
General Transcription Factors
Cyclins
RNA Polymerase II
Protein Sequence Analysis
Purification
Insects
Peptides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Revised Subunit Structure of Yeast Transcription Factor IIH (TFIIH) and Reconciliation with Human TFIIH. / Takagi, Yuichiro; Komori, Hirofumi; Chang, Wei Hau; Hudmon, Andy; Erdjument-Bromage, Hediye; Tempst, Paul; Kornberg, Roger D.

In: Journal of Biological Chemistry, Vol. 278, No. 45, 07.11.2003, p. 43897-43900.

Research output: Contribution to journalArticle

Takagi, Yuichiro ; Komori, Hirofumi ; Chang, Wei Hau ; Hudmon, Andy ; Erdjument-Bromage, Hediye ; Tempst, Paul ; Kornberg, Roger D. / Revised Subunit Structure of Yeast Transcription Factor IIH (TFIIH) and Reconciliation with Human TFIIH. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 45. pp. 43897-43900.
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