Revised Subunit Structure of Yeast Transcription Factor IIH (TFIIH) and Reconciliation with Human TFIIH

Yuichiro Takagi, Hirofumi Komori, Wei Hau Chang, Andy Hudmon, Hediye Erdjument-Bromage, Paul Tempst, Roger D. Kornberg

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Tfb4 is identified as a subunit of the core complex of yeast RNA polymerase II general transcription factor IIH (TFIIH) by affinity purification, by peptide sequence analysis, and by expression of the entire complex in insect cells. Tfb3, previously identified as a component of the core complex, is shown instead to form a complex with cdk and cyclin subunits of TFIIH. This reassignment of subunits resolves a longstanding discrepancy between yeast and human TFIIH complexes.

Original languageEnglish (US)
Pages (from-to)43897-43900
Number of pages4
JournalJournal of Biological Chemistry
Volume278
Issue number45
DOIs
StatePublished - Nov 7 2003

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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