Rho-kinase regulates myosin II activation in MDCK cells during recovery after ATP depletion

T. A. Sutton, H. E. Mang, S. J. Atkinson

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Alterations in the actin cytoskeleton of renal tubular epithelial cells during periods of ischemic injury and recovery have important consequences for normal cell and kidney function. Myosin II has been demonstrated to be an important effector in organizing basal actin structures in some cell types. ATP depletion in vitro has been demonstrated to recapitulate alterations of the actin cytoskeleton in renal tubular epithelial cells observed during renal ischemia in vivo. We utilized this reversible cell culture model of ischemia to examine the correlation of the activation state and cellular distribution of myosin II with disruption of actin stress fibers in Madin-Darby canine kidney (MDCK) cells during ATP depletion and recovery from ATP depletion. We found that myosin II inactivation occurs rapidly and precedes dissociation of myosin II from actin stress fibers during ATP depletion. Myosin II activation temporally correlates with colocalization of myosin II to reorganizing stress fibers during recovery from ATP depletion. Furthermore, myosin activation and actin stress fiber formation were found to be Rho-associated Ser/Thr protein kinase dependent during recovery from ATP depletion.

Original languageEnglish (US)
Pages (from-to)F810-F818
JournalAmerican Journal of Physiology - Renal Physiology
Volume281
Issue number5 50-5
StatePublished - Dec 19 2001

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Myosin Type II
rho-Associated Kinases
Madin Darby Canine Kidney Cells
Stress Fibers
Adenosine Triphosphate
Actins
Kidney
Actin Cytoskeleton
Ischemia
Epithelial Cells
Myosins
Protein Kinases
Cell Culture Techniques
Wounds and Injuries

Keywords

  • Adenosine 5′-triphosphate depletion
  • Ischemic acute renal failure
  • Madin-Darby canine kidney cells
  • Myosin light chain kinase
  • Rho-associated serine/threonine protein kinase
  • Stress fibers

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Rho-kinase regulates myosin II activation in MDCK cells during recovery after ATP depletion. / Sutton, T. A.; Mang, H. E.; Atkinson, S. J.

In: American Journal of Physiology - Renal Physiology, Vol. 281, No. 5 50-5, 19.12.2001, p. F810-F818.

Research output: Contribution to journalArticle

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