RhVEGF165 delivered in a porous β-tricalcium phosphate scaffold accelerates bridging of critical-sized defects in rabbit radii

Pei Yang, Chunsheng Wang, Zhibin Shi, Xin Huang, Xiaoqian Dang, Xudong Li, Shien Fong Lin, Kunzheng Wang

Research output: Contribution to journalArticle

12 Scopus citations


Segmental bone defects are a common obstacle in major orthopedic procedures, and the treatment of these defects remains a challenging clinical problem. Bone tissue engineering has been attracting much attention in recent years. We evaluated the ability of the specific combination of 3 μg rhVEGF165 with a novel porous β-tricalcium phosphate (b-TCP) scaffold coated with fibrin sealant (FS) to facilitate bone regeneration. Unilateral 15-mm long criticalsized defects were prepared in the radial diaphysis of rabbits and treated with rhVEGF165/FS/scaffold or FS/scaffold. Healing of the defects was assessed at 4, 8, and 12 weeks, radiologically, histologically, and biomechanically. The results of the study demonstrated that the critical-sized defects in the midshaft of the rabbit radius, treated with rhVEGF165 incorporated in porous β-TCP scaffold by FS, can be completely bridged by cortical bone in 12 weeks. The bone marrow space was also reformed histologically and radiologically at 12 weeks postsurgery in the rhVEGF165-treated group. Furthermore, biomechanical examination demonstrated that the segmental bone defects were not only radiologically and histologically repaired but were also mechanically repaired. Interestingly, none of the defects was completely repaired at 12 weeks following treatment with FS/ scaffold without rhVEGF165. A solution-driven process is likely the predominant mechanism of accelerating biodegradation of the β-TCP scaffold in the presence of rhVEGF 165; furthermore, cell-mediated phagocytosis also contributes to biodegradation of the biomaterials.

Original languageEnglish (US)
Pages (from-to)626-640
Number of pages15
JournalJournal of Biomedical Materials Research - Part A
Issue number2
StatePublished - Feb 1 2010


  • Adsorption
  • Biomaterial
  • Osteogenesis
  • Tissue engineering
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys

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