Right ventricular TNF resistance during endotoxemia: The differential effects on ventricular function

Troy A. Markel, Paul R. Crisostomo, Meijing Wang, Jeremy L. Herrmann, Aaron M. Abarbanell, Daniel R. Meldrum

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Right and left ventricular myocytes originate from different cellular progenitors; however, it is unknown whether these cells differ in their response to endotoxemia. We hypothesized that 1) the percentage of endotoxemic functional depression within the right ventricle (RV) would be smaller than that of the left ventricle; and 2) that better RV function would correlate with lower levels of right ventricular TNF production. Adult Sprague-Dawley rats were divided into right and left control and endotoxin groups. Controls received vehicle, while endotoxin groups received LPS at 20 mg/kg ip. Hearts were excised either 2 or 6 h after injection. Hearts excised at 2 h were assayed for TNF, IL-6, TNF receptor 1 (TNFR1), TNFR2, and via ELISA, while hearts excised at 6 h were assayed via the Langendorff model. The percentage of cardiac functional depression, exhibited as developed pressure, contractility, and rate of relaxation (expressed as a percentage of control) was significantly smaller in right ventricles compared with left ventricles following endotoxin exposure. Tissue levels of TNF were significantly elevated in both right and left ventricles 2 h after endotoxin exposure, and right ventricular endotoxin groups expressed higher levels of TNF compared with their left ventricular counterparts. No significant differences in IL-6, TNFR1, or TNFR2 levels were noted between endotoxin-exposed ventricles. This is the first study to demonstrate that right and left ventricular function differs after endotoxin exposure.

Original languageEnglish
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume293
Issue number5
DOIs
StatePublished - Nov 2007

Fingerprint

Endotoxemia
Ventricular Function
Endotoxins
Heart Ventricles
Receptors, Tumor Necrosis Factor, Type II
Interleukin-6 Receptors
Tumor Necrosis Factor Receptors
Right Ventricular Function
Left Ventricular Function
Muscle Cells
Sprague Dawley Rats
Enzyme-Linked Immunosorbent Assay
Pressure
Control Groups
Injections

Keywords

  • Cardiac
  • Cardiovascular collapse
  • IL-6
  • Sepsis

ASJC Scopus subject areas

  • Physiology

Cite this

Right ventricular TNF resistance during endotoxemia : The differential effects on ventricular function. / Markel, Troy A.; Crisostomo, Paul R.; Wang, Meijing; Herrmann, Jeremy L.; Abarbanell, Aaron M.; Meldrum, Daniel R.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 293, No. 5, 11.2007.

Research output: Contribution to journalArticle

Markel, Troy A. ; Crisostomo, Paul R. ; Wang, Meijing ; Herrmann, Jeremy L. ; Abarbanell, Aaron M. ; Meldrum, Daniel R. / Right ventricular TNF resistance during endotoxemia : The differential effects on ventricular function. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2007 ; Vol. 293, No. 5.
@article{90680732b36144eaa416e56bb9175c64,
title = "Right ventricular TNF resistance during endotoxemia: The differential effects on ventricular function",
abstract = "Right and left ventricular myocytes originate from different cellular progenitors; however, it is unknown whether these cells differ in their response to endotoxemia. We hypothesized that 1) the percentage of endotoxemic functional depression within the right ventricle (RV) would be smaller than that of the left ventricle; and 2) that better RV function would correlate with lower levels of right ventricular TNF production. Adult Sprague-Dawley rats were divided into right and left control and endotoxin groups. Controls received vehicle, while endotoxin groups received LPS at 20 mg/kg ip. Hearts were excised either 2 or 6 h after injection. Hearts excised at 2 h were assayed for TNF, IL-6, TNF receptor 1 (TNFR1), TNFR2, and via ELISA, while hearts excised at 6 h were assayed via the Langendorff model. The percentage of cardiac functional depression, exhibited as developed pressure, contractility, and rate of relaxation (expressed as a percentage of control) was significantly smaller in right ventricles compared with left ventricles following endotoxin exposure. Tissue levels of TNF were significantly elevated in both right and left ventricles 2 h after endotoxin exposure, and right ventricular endotoxin groups expressed higher levels of TNF compared with their left ventricular counterparts. No significant differences in IL-6, TNFR1, or TNFR2 levels were noted between endotoxin-exposed ventricles. This is the first study to demonstrate that right and left ventricular function differs after endotoxin exposure.",
keywords = "Cardiac, Cardiovascular collapse, IL-6, Sepsis",
author = "Markel, {Troy A.} and Crisostomo, {Paul R.} and Meijing Wang and Herrmann, {Jeremy L.} and Abarbanell, {Aaron M.} and Meldrum, {Daniel R.}",
year = "2007",
month = "11",
doi = "10.1152/ajpregu.00359.2007",
language = "English",
volume = "293",
journal = "American Journal of Physiology",
issn = "0193-1857",
publisher = "American Physiological Society",
number = "5",

}

TY - JOUR

T1 - Right ventricular TNF resistance during endotoxemia

T2 - The differential effects on ventricular function

AU - Markel, Troy A.

AU - Crisostomo, Paul R.

AU - Wang, Meijing

AU - Herrmann, Jeremy L.

AU - Abarbanell, Aaron M.

AU - Meldrum, Daniel R.

PY - 2007/11

Y1 - 2007/11

N2 - Right and left ventricular myocytes originate from different cellular progenitors; however, it is unknown whether these cells differ in their response to endotoxemia. We hypothesized that 1) the percentage of endotoxemic functional depression within the right ventricle (RV) would be smaller than that of the left ventricle; and 2) that better RV function would correlate with lower levels of right ventricular TNF production. Adult Sprague-Dawley rats were divided into right and left control and endotoxin groups. Controls received vehicle, while endotoxin groups received LPS at 20 mg/kg ip. Hearts were excised either 2 or 6 h after injection. Hearts excised at 2 h were assayed for TNF, IL-6, TNF receptor 1 (TNFR1), TNFR2, and via ELISA, while hearts excised at 6 h were assayed via the Langendorff model. The percentage of cardiac functional depression, exhibited as developed pressure, contractility, and rate of relaxation (expressed as a percentage of control) was significantly smaller in right ventricles compared with left ventricles following endotoxin exposure. Tissue levels of TNF were significantly elevated in both right and left ventricles 2 h after endotoxin exposure, and right ventricular endotoxin groups expressed higher levels of TNF compared with their left ventricular counterparts. No significant differences in IL-6, TNFR1, or TNFR2 levels were noted between endotoxin-exposed ventricles. This is the first study to demonstrate that right and left ventricular function differs after endotoxin exposure.

AB - Right and left ventricular myocytes originate from different cellular progenitors; however, it is unknown whether these cells differ in their response to endotoxemia. We hypothesized that 1) the percentage of endotoxemic functional depression within the right ventricle (RV) would be smaller than that of the left ventricle; and 2) that better RV function would correlate with lower levels of right ventricular TNF production. Adult Sprague-Dawley rats were divided into right and left control and endotoxin groups. Controls received vehicle, while endotoxin groups received LPS at 20 mg/kg ip. Hearts were excised either 2 or 6 h after injection. Hearts excised at 2 h were assayed for TNF, IL-6, TNF receptor 1 (TNFR1), TNFR2, and via ELISA, while hearts excised at 6 h were assayed via the Langendorff model. The percentage of cardiac functional depression, exhibited as developed pressure, contractility, and rate of relaxation (expressed as a percentage of control) was significantly smaller in right ventricles compared with left ventricles following endotoxin exposure. Tissue levels of TNF were significantly elevated in both right and left ventricles 2 h after endotoxin exposure, and right ventricular endotoxin groups expressed higher levels of TNF compared with their left ventricular counterparts. No significant differences in IL-6, TNFR1, or TNFR2 levels were noted between endotoxin-exposed ventricles. This is the first study to demonstrate that right and left ventricular function differs after endotoxin exposure.

KW - Cardiac

KW - Cardiovascular collapse

KW - IL-6

KW - Sepsis

UR - http://www.scopus.com/inward/record.url?scp=36048938940&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36048938940&partnerID=8YFLogxK

U2 - 10.1152/ajpregu.00359.2007

DO - 10.1152/ajpregu.00359.2007

M3 - Article

C2 - 17715182

AN - SCOPUS:36048938940

VL - 293

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0193-1857

IS - 5

ER -