Risedronate and alendronate suppress osteocyte apoptosis following cyclic fatigue loading

Helene Follet, Jiliang Li, Roger J. Phipps, Siu Hui, Keith Condon, David Burr

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Purpose: The purpose of this study was to determine whether bisphosphonate treatment can prevent or delay osteocyte apoptosis in a cyclic fatigue animal model and if there are differences between two different bisphosphonates in their effects on osteocyte apoptosis. Introduction: Fatigue loading induces microdamage in long bones in rats and causes osteocyte apoptosis. In vitro data suggest that the bisphosphonates can prevent osteocyte apoptosis. Materials and methods: Six month old female Sprague-Dawley rats (n = 72) were given a daily subcutaneous (sc) injection of saline vehicle, risedronate (RIS: 0.05 μg/kg per day) or alendronate (ALN: 0.1 μg/kg per day). On the 8th day of drug treatment, an axial compressive load was applied to the right ulna using a load-controlled electromagnetic device (17N, 6000 cycles, 2 Hz, 10% loss of stiffness ∼ 1 h). Three, seven or ten days after loading, the animals were sacrificed. Immunohistochemistry for caspase-3 was performed to assess the extent of osteocyte apoptosis in loaded and non-loaded ulnas. Results: Microdamage (Mdx) created by cyclic loading of the ulna induced a significant increase (p = 0.03) in the number of apoptotic osteocytes compared to non-damaged regions of the same ulna, and compared to the contralateral non-loaded ulna. Risedronate and alendronate had an early effect (3 days after loading) on reducing load-induced osteocyte apoptosis. Risedronate significantly reduced the density of apoptotic osteocytes compared to vehicle-treated controls by ∼ 50% in the Mdx area, whereas alendronate reduced it by ∼ 40%. There were no differences among groups by seven days following loading. Conclusions: (1) Low doses of risedronate or alendronate suppressed osteocyte apoptosis induced by fatigue loading of the ulna in rats. (2) There was no difference between the effects of risedronate or alendronate on osteocyte apoptosis at these doses.

Original languageEnglish
Pages (from-to)1172-1177
Number of pages6
JournalBone
Volume40
Issue number4
DOIs
StatePublished - Apr 2007

Fingerprint

Alendronate
Osteocytes
Fatigue
Ulna
Apoptosis
Diphosphonates
Risedronate Sodium
Electromagnetic Phenomena
Subcutaneous Injections
Caspase 3
Sprague Dawley Rats
Animal Models
Immunohistochemistry
Bone and Bones

Keywords

  • Apoptosis
  • Bisphosphonates
  • Mechanical loading
  • Microdamage
  • Osteocytes

ASJC Scopus subject areas

  • Physiology
  • Hematology

Cite this

Risedronate and alendronate suppress osteocyte apoptosis following cyclic fatigue loading. / Follet, Helene; Li, Jiliang; Phipps, Roger J.; Hui, Siu; Condon, Keith; Burr, David.

In: Bone, Vol. 40, No. 4, 04.2007, p. 1172-1177.

Research output: Contribution to journalArticle

Follet, Helene ; Li, Jiliang ; Phipps, Roger J. ; Hui, Siu ; Condon, Keith ; Burr, David. / Risedronate and alendronate suppress osteocyte apoptosis following cyclic fatigue loading. In: Bone. 2007 ; Vol. 40, No. 4. pp. 1172-1177.
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abstract = "Purpose: The purpose of this study was to determine whether bisphosphonate treatment can prevent or delay osteocyte apoptosis in a cyclic fatigue animal model and if there are differences between two different bisphosphonates in their effects on osteocyte apoptosis. Introduction: Fatigue loading induces microdamage in long bones in rats and causes osteocyte apoptosis. In vitro data suggest that the bisphosphonates can prevent osteocyte apoptosis. Materials and methods: Six month old female Sprague-Dawley rats (n = 72) were given a daily subcutaneous (sc) injection of saline vehicle, risedronate (RIS: 0.05 μg/kg per day) or alendronate (ALN: 0.1 μg/kg per day). On the 8th day of drug treatment, an axial compressive load was applied to the right ulna using a load-controlled electromagnetic device (17N, 6000 cycles, 2 Hz, 10{\%} loss of stiffness ∼ 1 h). Three, seven or ten days after loading, the animals were sacrificed. Immunohistochemistry for caspase-3 was performed to assess the extent of osteocyte apoptosis in loaded and non-loaded ulnas. Results: Microdamage (Mdx) created by cyclic loading of the ulna induced a significant increase (p = 0.03) in the number of apoptotic osteocytes compared to non-damaged regions of the same ulna, and compared to the contralateral non-loaded ulna. Risedronate and alendronate had an early effect (3 days after loading) on reducing load-induced osteocyte apoptosis. Risedronate significantly reduced the density of apoptotic osteocytes compared to vehicle-treated controls by ∼ 50{\%} in the Mdx area, whereas alendronate reduced it by ∼ 40{\%}. There were no differences among groups by seven days following loading. Conclusions: (1) Low doses of risedronate or alendronate suppressed osteocyte apoptosis induced by fatigue loading of the ulna in rats. (2) There was no difference between the effects of risedronate or alendronate on osteocyte apoptosis at these doses.",
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AU - Condon, Keith

AU - Burr, David

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N2 - Purpose: The purpose of this study was to determine whether bisphosphonate treatment can prevent or delay osteocyte apoptosis in a cyclic fatigue animal model and if there are differences between two different bisphosphonates in their effects on osteocyte apoptosis. Introduction: Fatigue loading induces microdamage in long bones in rats and causes osteocyte apoptosis. In vitro data suggest that the bisphosphonates can prevent osteocyte apoptosis. Materials and methods: Six month old female Sprague-Dawley rats (n = 72) were given a daily subcutaneous (sc) injection of saline vehicle, risedronate (RIS: 0.05 μg/kg per day) or alendronate (ALN: 0.1 μg/kg per day). On the 8th day of drug treatment, an axial compressive load was applied to the right ulna using a load-controlled electromagnetic device (17N, 6000 cycles, 2 Hz, 10% loss of stiffness ∼ 1 h). Three, seven or ten days after loading, the animals were sacrificed. Immunohistochemistry for caspase-3 was performed to assess the extent of osteocyte apoptosis in loaded and non-loaded ulnas. Results: Microdamage (Mdx) created by cyclic loading of the ulna induced a significant increase (p = 0.03) in the number of apoptotic osteocytes compared to non-damaged regions of the same ulna, and compared to the contralateral non-loaded ulna. Risedronate and alendronate had an early effect (3 days after loading) on reducing load-induced osteocyte apoptosis. Risedronate significantly reduced the density of apoptotic osteocytes compared to vehicle-treated controls by ∼ 50% in the Mdx area, whereas alendronate reduced it by ∼ 40%. There were no differences among groups by seven days following loading. Conclusions: (1) Low doses of risedronate or alendronate suppressed osteocyte apoptosis induced by fatigue loading of the ulna in rats. (2) There was no difference between the effects of risedronate or alendronate on osteocyte apoptosis at these doses.

AB - Purpose: The purpose of this study was to determine whether bisphosphonate treatment can prevent or delay osteocyte apoptosis in a cyclic fatigue animal model and if there are differences between two different bisphosphonates in their effects on osteocyte apoptosis. Introduction: Fatigue loading induces microdamage in long bones in rats and causes osteocyte apoptosis. In vitro data suggest that the bisphosphonates can prevent osteocyte apoptosis. Materials and methods: Six month old female Sprague-Dawley rats (n = 72) were given a daily subcutaneous (sc) injection of saline vehicle, risedronate (RIS: 0.05 μg/kg per day) or alendronate (ALN: 0.1 μg/kg per day). On the 8th day of drug treatment, an axial compressive load was applied to the right ulna using a load-controlled electromagnetic device (17N, 6000 cycles, 2 Hz, 10% loss of stiffness ∼ 1 h). Three, seven or ten days after loading, the animals were sacrificed. Immunohistochemistry for caspase-3 was performed to assess the extent of osteocyte apoptosis in loaded and non-loaded ulnas. Results: Microdamage (Mdx) created by cyclic loading of the ulna induced a significant increase (p = 0.03) in the number of apoptotic osteocytes compared to non-damaged regions of the same ulna, and compared to the contralateral non-loaded ulna. Risedronate and alendronate had an early effect (3 days after loading) on reducing load-induced osteocyte apoptosis. Risedronate significantly reduced the density of apoptotic osteocytes compared to vehicle-treated controls by ∼ 50% in the Mdx area, whereas alendronate reduced it by ∼ 40%. There were no differences among groups by seven days following loading. Conclusions: (1) Low doses of risedronate or alendronate suppressed osteocyte apoptosis induced by fatigue loading of the ulna in rats. (2) There was no difference between the effects of risedronate or alendronate on osteocyte apoptosis at these doses.

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